miR-140靶向LRP4基因对甲状腺癌细胞增殖和迁移的影响  

Effect of miR-140 Targeting LRP4 Gene on Proliferation and Migration of Thyroid Cancer Cells

作　　者：许华[1] 路亮 XU Hua;LU Liang(The Third Affiliated Hospital to Henan University of Chinese Medicine,Zhengzhou Henan China 450008;Zhengzhou Hospital of Chinese Medicine,Zhengzhou Henan China 450007)

机构地区：[1]河南中医药大学第三附属医院,河南郑州450008 [2]郑州市中医院,河南郑州450007

出　　处：《中医学报》2019年第10期2175-2180,共6页Acta Chinese Medicine

摘　　要：目的:探讨miR-140对甲状腺癌细胞增殖、迁移和侵袭的影响以及潜在的作用机制。方法:运用qRT-PCR检测甲状腺癌TPC-1细胞和正常甲状腺细胞Nthy-ori 3-1中miR-140和LRP4的表达水平;将miR-con组(转染miR-con)、miR-140组(转染miR-140 mimics)、si-con组(转染si-con)、si-LRP4组(转染pcDNA-LRP4)、anti-miR-con组(转染anti-miR-con)、anti-miR-140组(转染anti-miR-140)、miR-con+WT-LRP4组(转染miR-con和WT-LRP4)、miR-con+MUT-LRP4组(转染miR-con和MUT-LRP4)、miR-140+WT-LRP4组(转染miR-140 mimics和WT-LRP4)、miR-140+MUT-LRP4组(转染miR-140 mimics和MUT-LRP4)、miR-140+pcDNA组(转染miR-140 mimics和pcDNA)、miR-140+pcDNA-LRP4组(转染miR-140 mimics和pcDNA-LRP4)均用脂质体法转染至TPC-1细胞;采用CCK-8法检测各组细胞增殖;Transwell检测各组细胞迁移和侵袭;双荧光素酶报告基因检测实验检测荧光活性。结果:甲状腺癌TPC-1细胞在正常甲状腺细胞Nthy-ori 3-1、miR-140的表达水平显著降低,LRP4 mRNA和蛋白质的表达水平显著升高(P<0.05);过表达miR-140、抑制表达LRP4均可抑制TPC-1细胞增殖、迁移和侵袭;miR-140可靶向负调控LRP4的表达。过表达LRP4可逆转miR-140过表达对甲状腺癌TPC-1细胞增殖、迁移和侵袭的抑制作用。结论:miR-140可抑制甲状腺癌TPC-1细胞的增殖、迁移和侵袭,其机制可能与靶向LRP4有关,将可为甲状腺癌的诊断、治疗提供新靶点。Objective:To investigate the effects of miR-140 on proliferation,migration and invasion of thyroid cancer cells and the underlying mechanism of action.Methods:qRT-PCR was used to detect the expression levels of miR-140 and LRP4 in thyroid carcinoma TPC-1 cells and normal thyroid cells Nthy-ori 3-1;miR-con group(transfected miR-con) and miR-140 group(transfected with miR-140 mimics),si-con group(transfected with si-con),si-LRP4 group(transfected with pcDNA-LRP4),anti-miR-con group(transfected with anti-miR-con),anti-miR-140(transfected anti-miR-140),miR-con+WT-LRP4(transfected miR-con and WT-LRP4),miR-con+MUT-LRP4(transfected with miR-con and MUT-LRP4),miR-140+WT-LRP4(transfected with miR-140 mimics and WT-LRP4),miR-140+MUT-LRP4(transfected with miR-140 mimics and MUT-LRP4),miR-140 The+pcDNA group(transfected with miR-140 mimics and pcDNA) and the miR-140+pcDNA-LRP4 group(transfected with miR-140 mimics and pcDNA-LRP4) were transfected into TPC-1 cells by liposome method;CCK-8 method was used to detect the proliferation of each group;Transwell was used to detect the migration and invasion of each group;the dual luciferase reporter gene assay was used to detect the fluorescence activity.Results:The expression levels of Nthy-ori 3-1 and miR-140 in normal thyroid cells were significantly decreased,and the expression levels of LRP4 mRNA and protein were significantly increased(P<0.05).Overexpression of miR-140,Inhibition of expression of LRP4 can inhibit the proliferation,migration and invasion of TPC-1 cells;miR-140 can target the negative regulation of LRP4 expression.Overexpression of LRP4 reverses the inhibitory effect of miR-140 overexpression on proliferation,migration and invasion of thyroid carcinoma TPC-1 cells.Conclusion:miR-140 can inhibit the proliferation,migration and invasion of thyroid carcinoma TPC-1 cells.The mechanism may be related to targeting LRP4,which will provide a new target for the diagnosis and treatment of thyroid cancer.

关 键 词：甲状腺癌 miR-140 LRP4 增殖 迁移 侵袭 

分 类 号：R285.5[医药卫生—中药学]