固定化漆酶与Co(salen)/NaY组合催化转化木素的研究  

Study on Catalytic Degradation of Kraft Lignin by Immobilized Laccase/Co(salen)

作　　者：马雪梅 周学飞[1,2] MA Xue-mei;ZHOU Xue-fei(Faculty of Chemical Engineering,Kunming University of Science and Technology,Kunming 650500,China;State Key Laboratory of Non Food Biomass and Enzyme Technology,Nanning 530226,China)

机构地区：[1]昆明理工大学化学工程学院,云南昆明650500 [2]非粮生物质酶解国家重点实验室,广西南宁530226

出　　处：《纤维素科学与技术》2019年第3期1-7,共7页Journal of Cellulose Science and Technology

基　　金：国家自然科学基金（21766015）;昆明理工大学分析测试基金（2018M20172208046）;非粮生物质酶解国家重点实验室开放基金（2018-15-Z03-10）

摘　　要：把固定化漆酶和Co(salen)/NaY两种催化剂应用于一锅法组合催化转化木素。通过正交和单因素实验对膨润土固定化漆酶和Co(salen)/NaY催化转化木素的工艺进行探讨,结果显示,两种催化剂组合催化效果比不加催化剂或单独催化好,组合催化最佳工艺为:木素用量100 mg,催化剂比例1∶4,反应时间3 h,H2O2用量4mL,催化剂总用量8 mg,pH8.5。通过GC-MS技术对芳香类木素降解产物进行表征,结果显示,芳香类木素降解产物主要为香草醛,其他为丁香醛、2-甲氧基苯酚、4-羟基-3-甲氧基苯乙酮、4-羟基苯甲醛、4-乙酰氧基苯甲醛、2,6-二甲氧基苯酚、4-羟基-3,5-二甲氧基苯甲酸、2-羟基-6-甲氧基苯乙酮、1,2-二苯乙烯。一锅法组合催化显著增强了香草醛的产生。The transformation of lignin using immobilized laccase/Co(salen)/NaY catalysts in one-pot was studied.The process of lignin conversion with immobilized laccase/Co(salen)/NaY by orthogonal and single factor experiment were mainly investigated.The results showed that the catalytic effect of the combination of two catalysts was better than that without catalyst and alone.The optimal condition was found as follows:100 mg mass of lignin,1∶4 catalyst ratio,3 h reaction time,4 mL dosage of H2O2,8 mg total amount of catalyst,8.5 pH.In addition,the aromatic degradation products of lignin were characterized by GC-MS technique,and the results showed that vanillin was the most abundant compound among the analysed aromatic degradation products of lignin.The formation of syringaldehyde,2-methoxy phenol,4-hydroxy-3-methoxy acetophenone,4-hydroxy benzaldehyde,4-acetoxyl benzaldehyde,2,6-dimethoxy phenol,4-hydroxy-3,5-dimethoxy benzoic acid,2-hydroxy-6-methoxy acetophenone,1,2-diphenylethene was also found in this work.The yield of vanillin was significantly enhanced by the combined catalysis in one-pot.

关 键 词：硫酸盐木素 漆酶 Co(salen) 组合催化 降解产物 

分 类 号：TS713[轻工技术与工程—制浆造纸工程]