紧穗野生稻幼胚愈伤诱导、分化及生根苗的体系建立  

Establishment of Induction, Differentiation and Seedling Rooting System for Young Embryonic Calli of Oryza eichingeri

作　　者：王玲仙[1] 王波[1] 付坚[1] 邢佳鑫[1] 肖素勤[1] 张敦宇[1] 钟巧芳[1] 柯学[1] 陈玲[1] 殷富有[1] 蒋聪[1] 程在全[1] WANG Ling-xian;WANG Bo;FU Jian;XING Jia-xin;XIAO Su-qin;ZHANG Dun-yu;ZHONG Qiao-fang;KE Xue;CHEN Ling;YIN Fu-you;JIANG Cong;CHENG Zai-quan(Biotechnology and Germplasm Resources Institute,Yunnan Academy of Agricultural Sciences/Yunnan Provincial Key Lab of Agricultural Biotechnology/Key Lab of Southwestern Crop Gene Resources and Germplasm Innovation,Ministry of Agriculture and Rural Affairs,Kunming 650205,China)

机构地区：[1]云南省农业科学院生物技术与种质资源研究所/云南省农业生物技术重点实验室/农业农村部西南作物基因资源与种质创制重点实验室

出　　处：《江西农业学报》2019年第10期10-15,共6页Acta Agriculturae Jiangxi

基　　金：云南省农业联合重点项目[2017FG001(-007)];云南省农业基础研究联合专项面上项目[2017FG001(-023)]

摘　　要：以紧穗野生稻幼胚为实验材料,采用N6和MS为基本培养基,研究不同浓度激素配比对紧穗野生稻幼胚愈伤诱导、分化和生根的影响。结果表明,以N6为基本培养基,2,4-D浓度为5.0 mg/L的培养基诱导出愈率最高,达到100%。以N6为基本培养基,6-BA浓度为4.0 mg/L,添加NAA 0.3 mg/L、KT 2.0 mg/L、ZT 2.0 mg/L的培养基分化率最高,达到88%。较好的胚性愈伤在最适合分化的培养基中能够获得较高的分化率,以N6为基本培养基,IAA浓度为0.3 mg/L的培养基生根率最高,达到96%。Using young embryos of Oryza eichingeri as experimental materials,N 6 and MS as the basic medium,we systematically studied the effects of different concentrations of hormone ratios on callus induction,differentiation and rooting of young embryos.The results showed that the induction rate of the callus on the medium with N 6 as the basic medium and the 2,4-D concentration of 5.0 mg/L reached the highest(100%),whereas the differentiation rate on N 6 basic medium with 4.0 mg/L 6-BA,0.3 mg/L NAA,2.0 mg/L KT and 2.0 mg/L ZT reached the highest(88%).The better embryonic callus had high differentiation rate on the appropriate medium,and the rooting rate on N 6 basic medium with 0.3 mg/L IAA reached the highest(96%).

关 键 词：紧穗野生稻 幼胚 组培 

分 类 号：S511[农业科学—作物学]