miR-92a-3p对TNF-α,IL-6和DcR3的影响  

Effects of miRNA-92a-3p on TNF-α,IL-6 and DcR3

作　　者：陈洪卫[1] 娄晓丽[1] 赵静静[1] 侯彦强[1] CHEN Hongwei;LOU Xiaoli;ZHAO Jingjing;HOU Yanqiang(Department of Clinical Laboratory,Shanghai Songjiang District Central Hospital,Shanghai 201600,China)

机构地区：[1]上海市松江区中心医院检验科

出　　处：《国际检验医学杂志》2019年第20期2444-2448,共5页International Journal of Laboratory Medicine

基　　金：上海市卫健委青年科研项目(20164y0025);上海市松江区科学技术攻关项目(2017sjkjgg48)

摘　　要：目的探讨miR-92a-3p对肿瘤坏死因子-α(TNF-α),白细胞介素-6(IL-6)和诱导因子3(DcR3)的影响。方法用人急性单核细胞白血病细胞(THP-1)设置阴性对照组(NC)。用1μg/mL LPS分别处理THP-1细胞6 h和24 h,实时荧光定量聚合酶链反应(q-PCR)检测TNF-α,IL-6和DcR3的表达,作为Sepsis组(NC+LPS)。利用1μg/mL的LPS处理过表达miR-92a-3p的THP-1细胞,作为过表达miR-92a-3p的Sepsis组(miR-92a-3p+LPS)。利用q-PCR,酶联免疫吸附试验(ELISA)和Western blot分别检测各组TNF-α,IL-6和DcR3的表达情况。结果q-PCR,ELISA和Western blot检测结果显示,TNF-α和IL-6在LPS组、miR-92a-3p+LPS组和NC组的相对表达量均呈下调趋势,差异有统计学意义(P<0.05),DcR3表达差异无统计学意义(P>0.05)。结论miR-92a-3p可抑制TNF-α和IL-6的释放,具有抗炎作用,但是其对DcR3的作用不显著。Objective To investigate the effects of miRNA-92a-3p on tumor necrosis factor-alpha(TNF-α),interleukin-6(IL-6)and decoy factor-3(DcR3).Methods Human acute mononuclear leukemia cells(THP-1)were used as negative control group(NC).THP-1 cells were treated with 1μg/mL LPS for 6 h and 24 h,respectively.The expression of TNF-a,IL-6 and DcR3 was detected by q-PCR as Sepsis group(NC+LPS).THP-1 cells overexpressing miRNA-92a-3p were treated with 1μg/mL LPS as sepsis group overexpressing miRNA-92a-3p(miRNA-92a-3p+LPS).The expression of TNF-α,IL-6 and DcR3 was detected by q-PCR,ELISA and Western blot.Results The results of q-PCR,ELISA and Western blot showed that the relative expression of TNF-αand IL-6 in LPS group,Mi-92a-3p+LPS group and NC group decreased(P<0.05),while the expression of DcR3 had no statistical significance(P>0.05).Conclusion MiR-92a-3p can inhibit the release of TNF-αand IL-6,and has anti-inflammatory effect,but its effect on DcR3 is not significant.

关 键 词：脓毒症 miR-92a-3p 肿瘤坏死因子-Α 白细胞介素-6 诱导因子3 

分 类 号：R446.1[医药卫生—诊断学]