pH和近红外光双响应的包裹二硫化钼纳米片和阿霉素的金属-有机框架ZIF-8用于肿瘤化学/光热协同治疗  

pH- and NIR Laser Dual-Responsive Metal-Organic Frameworks ZIF-8 with MoS2 Nanosheets and DOX Loading for Chemo/Photothermal Synergistic Cancer Therapy

作　　者：李璟[1] 彭倩[1] 王丽姣 王晨旭[1] 杨凡[1] LI Jing;PENG Qian;WANG Lijiao;WANG Chenxu;YANG Fan(Institute of Geriatric,People’s Hospital of Hunan Province,Changsha 410016,China)

机构地区：[1]湖南省人民医院老年医学研究所

出　　处：《激光生物学报》2019年第5期421-430,共10页Acta Laser Biology Sinica

基　　金：湖南省中医药管理局科研计划项目(201866)

摘　　要：利用金属-有机框架材料ZIF-8包裹二硫化钼(MoS 2)纳米片和阿霉素(DOX)构建一种可通过酸性pH和近红外(NIR)光双触发的肿瘤化学/光热协同治疗体系。首先,通过水热反应和超声处理制备粒径为~100 nm、厚度为0.3~1.4 nm的MoS 2纳米片。然后,通过一步法将可酸降解的金属-有机框架ZIF-8包裹在所制备的MoS 2纳米片上,并同时装载抗肿瘤药物DOX,形成装载DOX的ZIF-8包裹MoS 2纳米复合物(DOX/MoS 2@ZIF-8)。将该纳米复合物应用到肿瘤细胞的化学/光热协同治疗:当处于酸性条件(例如:溶酶体中pH大约为5)和NIR激光(780 nm,2.1 W/cm 2)照射的情况下,DOX/MoS 2@ZIF-8纳米复合物上包裹的ZIF-8金属-有机框架会发生酸降解,释放出所包裹的DOX,细胞质中的DOX可以进入细胞核中诱导细胞凋亡;同时,MoS 2纳米片能够将光能转换为热能,光致高温同样能诱导细胞凋亡,因此,化学/光热协同肿瘤治疗得以实现。细胞存活率试验证明:该DOX/MoS 2@ZIF-8纳米复合物在SMMC-7721细胞上表现出良好的化学/光热协同治疗作用,能够对肿瘤细胞进行高效地杀伤。Here,we report a novel metal-organic framework ZIF-8-based nanocomposite with encapsulated molybdenum disulfide(MoS 2)nanosheets and doxorubicin(DOX)for pH-and NIR-triggered synergistic chemo/photothermal treatment of cancer cells.First,MoS 2 nanosheets,which have uniform size of^100 nm and topological height of 0.3~1.4 nm,are synthesized by hydrothermal reaction and ultrasonic treatment.Subsequently,an acid-degradable metal-organic framework ZIF-8 is employed to synchronously encapsulate MoS 2 nanosheets and anti-cancer drug DOX to obtain DOX loaded ZIF-8 coated MoS 2(DOX/MoS 2@ZIF-8)nanocomposites by one-step method.The nanocomposites are used for chemo/photothermal synergistic treatment of cancer cells:under acid conditions(e.g.pH 5.0 in a lysosome)and NIR laser irradiation(780 nm,2.1 W/cm 2),the ZIF-8 of the DOX/MoS 2@ZIF-8 nanocomposites can be degraded,resulting in the release of encapsulated DOX,the cytosolic DOX can enter the nucleus to induce apoptosis.Meanwhile,the MoS 2 nanosheets can effectively convert NIR laser light energy into heat energy,the photoinduced hyperthermia can also induce apoptosis.Thus,chemo/photothermal synergistic cancer therapy is realized.Cell viability assays show that this DOX/MoS 2@ZIF-8 nanocomposites have an excellent chemo/photothermal synergistic treatment effect on SMMC-7721 cells,which can kill the tumor cells efficiently.

关 键 词：二硫化钼纳米片 金属-有机框架材料 类沸石咪唑酯骨架材料-8 化学/光热协同治疗 酸/近红外光双响应的药物释放 

分 类 号：O657[理学—分析化学]