外源性补充FGF-10对新生支气管肺发育不良小鼠肺损伤的修复作用研究  

Repair effect of exogenous supplementation of fibroblast growth factor-10 on lung injury in neonatal bronchopulmonary dysplasia mice

作　　者：王保兰 朱蓉[1] 王立新[1] WANG Bao-lan;ZHU Rong;WANG Li-xin(Department of Respiratory,the Affilated Huai'an No.1 People's Hospital of Nanjing Medical University,Huaian,Jiangsu 223300,China)

机构地区：[1]南京医科大学附属淮安第一医院呼吸科

出　　处：《临床肺科杂志》2019年第11期1986-1990,共5页Journal of Clinical Pulmonary Medicine

基　　金：江苏省卫生计生委医学科研课题立项(No H201554)

摘　　要：目的探究外源性补充纤维母细胞生长因子10(FGF-10)对新生支气管肺发育不良(BPD)小鼠肺损伤的修复作用。方法新生KM小鼠45只随机分为对照组、模型组、FGF-10组,每组15只,对照组置于空气中(氧浓度维持在21%),模型组、FGF-10组置于封闭氧箱中(氧浓度维持在60%),以制备BPD模型,从造模开始,FGF-10组小鼠给予外源性FGF-10 400ug/kg腹腔注射,1次/d,连续21 d。22 d时,观察小鼠一般情况,采用酶联免疫吸附法(ELISA)检测肺组织中炎性因子白细胞介素6(IL-6)、IL-8、肿瘤坏死因子-α(TNF-α)及FGF-10水平,采用Western Blot检测肺组织中核转录因子-κB(NF-κB)p65蛋白表达水平。结果FGF-10组小鼠较模型组小鼠生长、反应、进食、呼吸等一般状况好转,未出现死亡小鼠,各组小鼠体重差异显著(P<0.05)。FGF-10组病理改变较模型组明显减轻,肺大泡明显减少,肺泡结构趋于正常,炎性浸润、间质水肿有明显改善。模型组小鼠肺组织中FGF-10水平显著低于对照组(P<0.05);FGF-10组小鼠肺组织中FGF-10水平显著高于模型组(P<0.05);模型组小鼠肺组织中IL-6、IL-8、TNF-α、NF-κB p65水平显著高于对照组(P<0.05);FGF-10组小鼠肺组织中IL-6、IL-8、TNF-α、NF-κB p65水平显著低于模型组(P<0.05);FGF-10组小鼠肺组织中FGF-10、NF-κB p65、IL-6、IL-8、TNF-α水平与对照组比较有显著差异(P<0.05)。结论外源性补充FGF-10对新生支气管肺发育不良小鼠肺损伤具有修复作用,其作用可能通过抑制NF-κB信号通路介导的炎症反应而实现。Objective To investigate the repair effect of exogenous supplementation of fibroblast growth factor-10(FGF-10)on lung injury in neonatal bronchopulmonary dysplasia(BPD)mice.Methods 45 newborn KM mice were randomly divided into the control group,the model group and the FGF-10 group,with 15 rats in each group.The control group was placed in the air(oxygen concentration was maintained at 21%),and the model group and the FGF-10 group were placed in a closed oxygen chamber(oxygen concentration was maintained at 60%)to prepare BPD models.At the first day of modeling,the FGF-10 group was given exogenous FGF-10 400 ug/kg intraperitoneally,once a day for 21 days.On the 22nd day,the general condition of the mice was observed.The inflammatory factors interleukin-6(IL-6),IL-8,tumor necrosis factor-α(TNF-α)and FGF-10 levels were detected by enzyme-linked immunosorbent assay(ELISA).Western blot was used to detect the protein expression levels of nuclear transcription factor-κB(NF-κB)and p65 in lung tissue.Results Compared with the model group,the growth,reaction,feeding,and breathing of the mice in the FGF-10 group improved,and there was no dead mice.The body weight of each group was significantly different(P<0.05).The pathological changes of the FGF-10 group were significantly relieved compared with the model group,which showed reduced alveolar vesicles,normal alveolar structures,inflammatory infiltration and interstitial edema.The level of FGF-10 in the lung tissue ranking in a descending order was the FGF-10 group,the model group and the control group(P<0.05).The levels of IL-6,IL-8,TNF-αand NF-κB p65 in the lung tissue of the model group were significantly higher than those in the control group(P<0.05)and the FGF-10 group(P<0.05).The levels of FGF-10,NF-κB p65,IL-6,IL-8 and TNF-αin lung tissue of the FGF-10 group were significantly different from those in the control group(P<0.05).Conclusion Exogenous supplementation of FGF-10 has a repairing effect on lung injury in neonatal BPD mice,and its effect may be achieved

关 键 词：纤维母细胞生长因子10 支气管肺发育不良 肺损伤 损伤修复 NF-ΚB通路 

分 类 号：R28[医药卫生—中药学]