珍珠龙胆石斑鱼肠道枯草芽孢杆菌的分离鉴定及产酶能力分析  

Study on Bacillus subtilis from hybrid grouper (Epinephelus fuscoguttatus♀×Epinephelus lanceolatus♂) intestine and its zymogenicities

作　　者：王成强 王际英 黄炳山 李宝山 WANG Chengqiang;WANG Jiying;HUANG Bingshan;LI Baoshan(Shandong Key Laboratory of Marine Ecological Restoration,Shandong Marine Resource and Environment Research Institute,Yantai 264006,China)

机构地区：[1]山东省海洋资源与环境研究院山东省海洋生态修复重点实验室

出　　处：《渔业研究》2019年第5期366-373,共8页Journal of Fisheries Research

基　　金：烟台市科技计划项目(2016ZH068);山东省2018年度农业重大应用技术创新项目(201603003);2019年山东省自然科学基金项目(ZR2019PC061)

摘　　要：从健康珍珠龙胆石斑鱼肠道中分离纯化获得4株疑似枯草芽孢杆菌(Bacillus subtilis)菌株(B1、B2、B3和B4),并进行了菌落形态、菌株理化性质、16S rDNA序列和产酶能力分析等方面的研究。结果表明,在理化性质方面,4株菌的试验结果均符合枯草芽孢杆菌的生化特性,并结合菌落形态,初步证明4株分离菌可能均为枯草芽孢杆菌;同时,基因序列比对结果显示,4株菌的16S rDNA序列与已知的枯草芽孢杆菌序列相似性≥99%,最终鉴定均为枯草芽孢杆菌。经过产酶能力的试验研究发现,4株菌都具有产纤维素酶、淀粉酶和蛋白酶的能力,且B3菌株同其他3株菌相比,其产纤维素酶、淀粉酶和蛋白酶的能力均较强。因此,本试验选取B3作为具有益生菌潜力的菌株。Four suspected Bacillus strains were isolated from the intestine of healthy hybrid grouper,which were named as B1,B2,B3 and B4 and studied on the bacterial morphology,biochemistry characteristic,16S rDNA sequences and ectoenzyme-producing analysis.The results showed that four strains were consistent with Bacillus on biochemistry characteristic.Meanwhile,the 16S rDNA sequences of four strains were clustered at similarity levels(≥99%)with Bacillus.Finally,the results showed that four strains were all Bacillus.With the founded of ectoenzyme-producing experiment,four strains all had ectoenzyme-producing ability,such as cellulase,amylase and protease.And B3 strain had higher zymogenicities of cellulase,protease,and amylase than B1,B2 or B4.So this experiment finally selected B3 as a strain with potential probiotic.

关 键 词：珍珠龙胆石斑鱼 枯草芽孢杆菌 16S RDNA序列 产酶能力 

分 类 号：S963[农业科学—水产养殖]