竹节参总皂苷对高脂高糖小鼠脑组织凋亡的保护作用  

Protective Effect of Saponins from Panax japonicus on Brain Apoptosis in Mice with High-fat and High-fructose Diet

作　　者：王瑞 王婷[1] 张续蓝 望金欣 刘朝奇[1] 袁丁[1] 何毓敏[1] 周志勇[1] WANG Rui;WANG Ting;ZHANG Xu-lan;WANG Jin-xin;LIU Chao-qi;YUAN Ding;HE Yu-min;ZHOU Zhi-yong(College of Medical Science,China Three Gorges University,Yichang 443002,China)

机构地区：[1]三峡大学医学院

出　　处：《现代食品科技》2019年第10期25-31,共7页Modern Food Science and Technology

基　　金：三峡大学学位论文培优基金项目(2019SSPY106);国家自然科学基金项目(81374001;81673778)

摘　　要：观察竹节参总皂苷(saponins from Panax japonicas,SPJ)对高脂高糖饮食小鼠脑组织凋亡的保护作用。昆明小鼠随机分成正常对照组、高脂高糖模型组和药物低(100 mg/kg)、高(300 mg/kg)剂量组,分别给予普通饲料、高脂高糖饲料、竹节参总皂苷低、高剂量饲料喂养。Western blot法检测抑凋亡相关蛋白Bcl-2、促凋亡蛋白Bax、细胞色素C(Cytochrome C)、半胱天冬蛋白酶(Caspase-3和Caspase-12)、炎性因子诱导型一氧化氮合酶(inducible nitric oxide synthase,iNOS)、环氧合酶2(cyclooxygenase,COX-2)、IL-1β和NLRP3炎症小体包括NOD样受体家族3(NOD-like receptors,NLRP3)、凋亡相关斑点样蛋白(ASC)、Caspase-1和抗氧化应激蛋白沉默信息调节因子1(Silence information regulator 1,SIRT1)、腺苷酸活化蛋白激酶(Adenosine monophosphate activated protein kinase,AMPK)磷酸化水平。与高脂对照组相比,竹节参总皂苷高剂量组中Bcl-2、SIRT1和p-AMPK分别升高了1.31倍、1.05倍、1.33倍,Bax、Caspase 3、Caspase 12、Cytochrome C、COX-2、iNOS、IL-1β、NLRP3、Caspase 1和ASC分别下降了34%、39%、60%、37%、41%、25%、43%、49%、31%、52%。竹节参总皂苷对高脂高糖小鼠具有抗凋亡作用,其机制与其调节NLRP3炎症小体及炎性因子,增加抗氧化应激相关蛋白有关。The objective in this work was to investigate the effect of saponins from Panax japonicus(SPJ)on high-fat and high-fructose diet(HFFD)induced apoptosis in mice.KM mice were divided into normal control,high-fat and high-fructose-diet group,low-dose SPJ group(100 mg/kg)and high-dose SPJ group(300 mg/kg)and were fed with normal diet,high-fat and high-fructose diet(HFFD)and HFFD followed by SPJ at different doses(100 and 300 mg/kg)for one month,respectively.The protein levels of Bcl-2,Bax,Cytochrome C,Caspase-3,Caspase-12,COX-2,iNOS,IL-1β,NLRP3,Caspase-1,ASC,SIRT1 and p-AMPK were detected by western blot.The results showed that the protein levels of Bcl-2,SIRT1 and p-AMPK of high-dose SPJ group increased by 1.31,1.05 and 1.33 times,respectively.The protein levels of Bax、Caspase 3、Caspase 12、Cytochrome C、COX-2、iNOS、IL-1β、NLRP3、Caspase 1 and ASC decreased by 34%、39%、60%、37%、41%、25%、43%、49%、31%、52%,respectively.In conclusion,SPJ have strong anti-apoptosis effect on mice with high-fat and high-fructose diet,and its mechanism is related with inhibition of NLRP3 inflammasome activitation,resulting in a reduction of expression of inflammatory factors,or the upregulation of the proteins associated with oxidative stress.

关 键 词：竹节参总皂苷 高脂高糖饮食 凋亡 神经炎症 氧化应激 

分 类 号：R28[医药卫生—中药学]