弓形虫ROP16Ⅰ/Ⅲ蛋白对小鼠子宫基质细胞蜕膜化的影响  

Effect of Toxoplasma gondii ROP16Ⅰ/Ⅲ protein on decidualization of mouse uterine stromal cells

作　　者：金郁 计永胜[1] 姚湧[1] 汪学龙[1] Jin Yu;Ji Yongsheng;Yao Yong(Dept of Parasitology,School of Basic Medical Sciences,Anhui Medical University,Hefei 230032)

机构地区：[1]安徽医科大学基础医学院人体寄生虫教研室

出　　处：《安徽医科大学学报》2019年第10期1506-1510,共5页Acta Universitatis Medicinalis Anhui

基　　金：安徽高校自然科学研究项目(编号:KJ2016A353)

摘　　要：目的探讨弓形虫ROP16Ⅰ/Ⅲ蛋白对妊娠小鼠子宫基质细胞蜕膜化的影响。方法原代培养小鼠子宫基质细胞,用雌二醇、孕酮和cAMP体外诱导蜕膜化的同时加入ROP16重组慢病毒,72 h后qRT-PCR检测蜕膜化标志物催乳素(PRL)mRNA的表达,流式细胞术检测蜕膜细胞凋亡率的变化,并用Western blot检测细胞凋亡相关蛋白Bcl-2、Bax的表达水平。结果提取的小鼠子宫基质细胞纯度为95%以上,在体外诱导蜕膜化后,PRL mRNA的表达随时间延长而显著升高(P<0.05),在诱导的蜕膜细胞中过表达ROP16Ⅰ/Ⅲ,相比于对照组,蜕膜细胞的凋亡率降低(P<0.01),凋亡蛋白Bax表达降低,Bcl-2蛋白表达上调。结论弓形虫ROP16Ⅰ/Ⅲ蛋白会抑制蜕膜细胞的凋亡,可能会影响蜕膜细胞增殖与凋亡的平衡,从而影响胚胎的植入。Objective To investigate the effect of Toxoplasma gondii ROP16Ⅰ/Ⅲprotein on the decidualization of uterine stromal cells in pregnant mice.Methods Cultured primary mouse uterine stromal cells with estradiol and progesterone to induce decidualization in vitro,then infected with lentivirus.The expression of prolactin(PRL)mRNA was detected by qRT-PCR after 72 hours,and the apoptosis rate of decidual cells was detected by flow cytometry,Western blot was used to detect the expression of apoptosis-related protein Bcl-2 and Bax.Results The extracted mouse uterine stromal cells had a purity of over 95%.After induced decidualization in vitro,the expression of PRL mRNA increased significantly with time(P<0.05).Overexpression of ROP16Ⅰ/Ⅲwas induced in the induced decidual cells.Compared with the control group,the apoptosis rate of decidual cells was decreased(P<0.05),the expression of apoptosis protein Bax was significantly decreased,and the expression of Bcl-2 protein was up-regulated.Conclusion Toxoplasma gondii ROP16Ⅰ/Ⅲprotein inhibits apoptosis of decidual cells.It can affect the balance of proliferation and apoptosis of decidual cells and affect the implantation of embryos.

关 键 词：弓形虫 ROP16蛋白 蜕膜化 凋亡 

分 类 号：R382.33[医药卫生—医学寄生虫学]