镉诱导小白菜活性氧及抗氧化酶活性与自噬关系分析  

Relationship of Active Oxygen, Antioxidant Enzyme Activity and Autophagy under Cd Stress in Pakchoi

作　　者：肖旭峰[1] 李猛[1] 龙俊敏[2] 张明[2] 范淑英[1] 吴才君[1] XIAO Xu-feng;LI Meng;LONG Jun-min;Zhang Ming;FAN Shu-ying;WU Cai-jun(College of Agronomy,Jiangxi Agricultural University,Nanchang 330045,China;Jiangxi Biotech Vocational College,Nanchang 330200,China)

机构地区：[1]江西农业大学农学院,江西南昌330045 [2]江西生物科技职业学院,江西南昌330200

出　　处：《江西农业大学学报》2019年第5期873-880,共8页Acta Agriculturae Universitatis Jiangxiensis

基　　金：国家自然科学基金项目(31860560)~~

摘　　要：为探讨自噬在Cd胁迫小白菜生长中的作用,采用营养液培法研究了Cd胁迫下小白菜生长量、叶绿素、活性氧、MDA及抗氧化酶活性与自噬的关系。结果表明:随Cd^2+浓度增加,小白菜生长受到明显抑制,叶片褪绿,根系变短,并产生细胞自噬;叶绿素荧光参数Fm、Fv/Fm和Qp显著降低,而Qn、Y(NPQ)显著升高,SPAD值则“先升后降”;H2O2、O2^-·均呈上升趋势,差异显著,其中O2^-·增幅较H2O2大;SOD、CAT、APX及总抗坏血酸值为“先升后降”,而GSH-Px、ASA则持续上升,差异显著;根、叶中MDA含量升高,且叶比根过氧化损伤更为严重。由此,Cd应答可激活小白菜发生自噬;随Cd^2+浓度升高,ROS累积诱导自噬小体形成,然而自噬发生不足或过量都直接导致植株DNA损伤和程序性细胞死亡。In order to explore the effects of autophagy on the growth of Chinese cabbage under Cd stress,the relationship between growth,chlorophyll,reactive oxygen species,MDA,antioxidant enzyme activity and autophagy under Cd stress in Chinese cabbage was studied by a hydroponic experiment.The results showed that with the increase of Cd2+concentration,the growth of pakchoi was significantly inhibited,showing leaf chlorosis,root shortening and cell autophagy;the chlorophyll fluorescence parameters Fm,Fv/Fm and Qp decreased significantly,while Qn and Y(NPQ)increased significantly,and SPAD values increased first and then decreased;the increase of H2O2 and O2^-·was significant,and the increase of O2^-·was more dramatic than that of H2O2;SOD,CAT,APX and total antiserum acid value increased first and then decreased,while GSH-Px and ASA increased continuously with significant difference;the content of MDA in roots and leaves increased,and the damage to leaves was more serious than that to roots.In conclusion,Cd response is the cause of activation of autophagy in Chinese cabbage.With the increase of Cd2+concentration,ROS accumulation induced autophagosome formation.However,inadequate or excessive autophagy could directly lead to DNA damage and programmed cell death in plants.

关 键 词：小白菜 CD 活性氧 抗氧化酶 自噬 

分 类 号：S634.3[农业科学—蔬菜学]