挤压综合征中内质网应激及细胞凋亡的作用机制研究  

Study on the mechanism of endoplasmic reticulum stress and cell apoptosis in crush syndrome

作　　者：张雪梅 张凌 付平 ZHANG Xuemei;ZHANG Ling;FU Ping(The Second Affiliated Hospital of Chongqing Medical University,Chongqing 401120,China)

机构地区：[1]重庆医科大学附属第二医院

出　　处：《现代医药卫生》2019年第21期3294-3297,共4页Journal of Modern Medicine & Health

摘　　要：目的通过肌内注射甘油建立挤压综合征(CS)动物模型,分析研究内质网应激及细胞凋亡是否参与了CS的致病通路。方法将昆明小鼠随机分为对照组、CS组、N-乙酰半胱氨酸(NAC)组,每组10只,分别采取不同干预方式处理。连续观察48 h,记录小鼠一般状态和存活情况,绘制生存曲线。按上述相同处理方法将小鼠随机分为3组,每组20只,分别于处理后4、8、24、48 h麻醉并经心脏采血留取血液标本,开腹留取肾脏标本后立即处死小鼠。检测血清肌酐(SCr),采用免疫组织化学法、实时荧光定量PCR检测凋亡及内质网应激相关蛋白表达。结果CS组肾脏检测到的凋亡及内质网应激相关的标志蛋白表达高于对照组,而NAC组上述相关蛋白表达低于CS组,且生存率高于CS组,差异均有统计学意义(P<0.05)。结论内质网应激及凋亡参与了CS的致病机制,N-乙酰半胱氨酸在其中可能发挥了保护作用。Objective The animal model of crush syndrome(CS)was established by intramuscular injection of glycerol to analyze whether endoplasmic reticulum stress(ERS)and apoptosis were involved in the pathogenesis of CS.Methods The Kunming mice were randomly divided into the control group,CS group,N-acetylcysteine(NAC group),10 mice in each group,different intervention methods were adopted respectively.The mice were recorded for general condition and survival during 48 h and survival curve was then drafted.Also,other mice were randomly divided into 3 groups as previously described,20 mice in each group.The specimen of blood and kidneys were harvested at 4,8,24 h and 48 h respectively.The serum creatinine(SCr)was detected.Apoptosis and ERS-related protein expressions were detected by immunohistochemistry real-time quantitative PCR.Results Marker proteins of ERS and apoptosis were higher expressed in CS groups than those in control group,those proteins in NAC group were lower expressed than those in CS group,and the survival rate was higher in NAC group than that in CS group,the differences were statistically significant(P<0.05).Conclusion ERS and apoptosis participate in the pathogenesis of CS,and NAC may be an protective factor.

关 键 词：挤压综合征 急性肾损伤 内质网应激 细胞凋亡 

分 类 号：R392.9[医药卫生—免疫学]