灭活处理鸡新城疫疫苗替代流感病毒血凝及血凝抑制实验教学效果分析  

Study on the effect of inactivated new Newcastle vaccine serving as substitution of influenza virus on the hemagglutination assay and hemagglutination inhibition assay in experiment teaching

作　　者：胡涛[1] 张文昌 程正阳 李群[1] 王明丽[1] Hu Tao;Zhang Wenchang;Cheng Zhengyang(School of Basic Medical Sciences,Anhui Medical University,Hefei 230032)

机构地区：[1]安徽医科大学基础医学院

出　　处：《安徽医科大学学报》2019年第11期1817-1820,共4页Acta Universitatis Medicinalis Anhui

基　　金：安徽高校省级自然科学研究项目(编号:KJ2012ZD08)

摘　　要：比较灭活处理鸡新城疫疫苗株(La Sota株,简称鸡新城疫疫苗)替代流感病毒疫苗株(H 9N 2株,简称流感疫苗)的血凝(HA)及血凝抑制(HI)试验在实验教学中的应用。采用0.25%甲醛溶液灭活处理鸡新城疫疫苗后,分别对该疫苗的安全性及稳定性进行逐一检测,并与流感病毒同时进行HA及HI试验,以评价该灭活苗的相应HA及HI试验效果及两种病毒株试验结果的符合率。灭活处理的鸡新城疫疫苗安全性符合国家药典规定。该疫苗的血凝效价及其免疫血清的血凝抑制实验效价稳定,通过分批次抽取样品进行检验显示,抽检符合率为100%;教学实践中教学符合率>90%;符合替代流感病毒HA及HI试验的要求。采用0.25%甲醛溶液灭活处理后的鸡新城疫疫苗替代流感病毒的HA及HI试验,具有安全、稳定、易保存的特征;两者相应HA及HI试验结果一致,可作为相应微生物学实验教学替代材料。Substitution of influenza virus vaccine with inactivated Newcastle disease virus vaccine(La Sota Strain)in Hemagglutination assay and Hemagglutination inhibition assay,which were normal experiments in medical undergraduate training.The safety and stability of incativated Newcastle disease virus was tested by inoculated chicken embryonated eggs and hemagglutination assay respectively.The incativated Newcastle disease virus vaccine was safety,stable and was consistent to influenza virus in Hemagglutination assay and Hemagglutination inhibition assay with sampling compliance rate of 100%and teaching coincidence rate of more than 90%.Inactivated Newcastle disease virus with 0.25%formalin is safety,stability,excellent result and easy to save.It is an important substitution of influenza virus in microbiology teaching experiment.

关 键 词：流感病毒 鸡新城疫疫苗株 血凝及血凝抑制试验 甲醛灭活 教学效果 

分 类 号：R373.9[医药卫生—病原生物学]