UPLC测定健脾益肺口服液中白术内酯Ⅰ和白术内酯Ⅲ的含量  

Determination of Atractylenolide Ⅰ and Atractylenolide Ⅲ in Jianpi Yifei Oral Liquid by UPLC

作　　者：王丹丹[1] WANG Dan-dan(Department of Pharmacy,Nanjing Traditional Chinese Medicine Hospital,Nanjing University of Traditional Chinese Medicine,Nanjing 210001,Jiangsu,China)

机构地区：[1]南京市中医药大学附属南京市中医院药学部

出　　处：《医学信息》2019年第21期159-160,163,共3页Journal of Medical Information

基　　金：南京市药学会科研基金(编号：2018YX014)

摘　　要：目的使用UPLC测定健脾益肺口服液中白术内酯Ⅰ、白术内酯Ⅲ的含量。方法采用Waters H-class超高效液相色谱仪测定白术内酯Ⅰ、白术内酯Ⅲ的含量,色谱柱:Waters BEH C18(50 mm×2.1 mm,1.7μm),柱温:30℃,进样量:2μl,检测波长:220 nm,流动相:乙腈(A)-水(B),梯度洗脱,流速:0.2 ml/min。结果白术内酯Ⅰ线性方程为y=0.0528x+0.0241(r=0.9998),线性范围2~160μg/ml;白术内酯Ⅲ的线性方程为y=0.0786x(r=0.9998),线性范围3~140μg/ml;白术内酯Ⅰ及白术内酯Ⅲ的精密度、重复性、稳定性、加样回收率均符合要求。结论初步建立了健脾益肺口服液中白术内酯Ⅰ、白术内酯Ⅲ含量测定的高效、迅速的检测方法,为下一步质量标准的提高奠定基础。Objective To determine the content of atractylenolide Ⅰ and atractylenolide Ⅲ in Jianpi Yifei Oral Liquid by UPLC. Methods The contents of atractylenolide I and atractylenolide Ⅲ were determined by Waters H-class ultra performance liquid chromatography. Column: Waters BEH C18(50 mm × 2.1 mm, 1.7 μm), column temperature: 30°C, injection Amount: 2 μl, detection wavelength: 220 nm, mobile phase: acetonitrile(A)-water(B), gradient elution, flow rate: 0.2 ml/min. Results The linear equation of atractylenolide Ⅰ was y=0.0528 x+0.0241(r=0.9998), the linear range was 2~160 μg/ml;the linear equation of atractyl lactone Ⅲ was y=0.0786 x(r=0.9998), linear range 3 ~140 μg/ml;the precision, repeatability,stability and recovery rate of atractylenolide I and atractylenolide Ⅲ meet the requirements. Conclusion The high-efficiency and rapid detection method for the determination of atractylenolide I and atractylenolide Ⅲ in Jianpi Yifei Oral Liquid was established, which laid a foundation for the improvement of quality standards.

关 键 词：UPLC 白术内酯Ⅰ 白术内酯Ⅲ 

分 类 号：R284.1[医药卫生—中药学]