双歧杆菌对2型糖尿病大鼠血浆酪酪肽和胰岛素抵抗的影响  

Effect of bifidobacterium on plasma level of peptide YY and insulin resistance in type 2 diabetic rats

作　　者：吴道爱[1] 时照明[1] 杨青青[1] 吴晨辰[1] 丁言稳 马博[1] WU Dao-ai;SHI Zhao-ming;YANG Qing-qing;WU Chen-chen;DING Yan-wen;MA Bo(Department of Endocrinology,The First Affiliated Hospital of Bengbu MedicalCollege,Bengbu Anhui 233004,China)

机构地区：[1]蚌埠医学院第一附属医院内分泌科

出　　处：《蚌埠医学院学报》2019年第10期1313-1315,共3页Journal of Bengbu Medical College

基　　金：蚌埠医学院科技发展基金(BYKF1782)

摘　　要：目的:观察双歧杆菌对2型糖尿病(T2DM)大鼠血浆酪酪肽(PYY)和胰岛素抵抗指数(HOMA-IR)的影响。方法:30只周龄为9周SD雄性大鼠链脲佐菌素(STZ)造模后,根据双歧杆菌灌胃剂量分成4组:正常对照组(N组)、糖尿病组(T2DM组)、高剂量组和低剂量组,双歧杆菌连续灌胃6周后,测所有大鼠空腹血糖(FBS),处死后取血浆检测PYY、糖化血红蛋白(HbA1c)、空腹胰岛素(FINS)、血脂,计算HOMA-IR,比较4组大鼠上述指标的差异。结果:高剂量组和低剂量组HbA1c、FBS、FINS、HOMA-IR和三酰甘油低于T2DM组(P<0.01),但高于N组(P<0.05~P<0.01);高剂量组和低剂量组PYY高于T2DM组(P<0.01),但低于N组(P<0.01),高剂量组和低剂量组上述指标差异均无统计学意义(P>0.05),PYY和HOMA-IR呈负相关(r=-0.783,P<0.01)。结论:双歧杆菌可以降低T2DM大鼠HbA1c、FBS、FINS、TG和HOMA-IR,升高PYY。Objective:To observe the effects of bifidobacterium on the plasma level of peptide YY(YY)and HOMA-IR in type 2 diabetic(T2DM)rats.Methods:Thirty SD male STZ model rats aged 9 weeks were divided into the normal control group(group N),diabetes mellitus group(group T2DM),high dose group and low dose group according to the gavage dose of bifidobacteria.After 6 weeks of continuous bifidobacterium administration,the levels of fasting blood sugar(FBS)were measured in all rats.The serum levels of PYY,HbA1c,fasting insulin(FINS)and blood lipids in four groups were measured after death,the HOMA-IR was calculated to compare the differences of the above indexes among four groups.Results:The levels of HbA1c,FBS,FINS,HOMA-IR and triglyceride in high-dose group and low-dose group were significantly lower than those in group T2DM(P<0.01),and higher than those in group N(P<0.05 to P<0.01).The serum levels of PYY in high-dose group and low-dose group were significantly higher than that in group T2DM(P<0.01),but lower than that in group N(P<0.01).The differences of the above indictors between high-dose group and low-dose group were not statistically significant(P>0.05),and the level of PYY was negatively correlated with HOMA-IR(r=-0.783,P<0.01).Conclusions:Bifidobacterium can decrease the levels of HbA1c,FBS,FINS,TG and HOMA-IR,and increase the level of PYY in T2DM rats.

关 键 词：2型糖尿病 双歧杆菌 酪酪肽 胰岛素抵抗 大鼠 

分 类 号：R587.1[医药卫生—内分泌]