基于GBS、DArT-array和SSR标记构建普通小麦高密度遗传图谱  

Constructing high density genetic linkage map of bread wheat with GBS,DArT-array and SSR markers

作　　者：赵春杰 李慧慧[2] 刘德梅[3] 兰彩霞[1] ZHAO Chunjie;LI Huihui;LIU Demei;LAN Caixia(College of Plant Science&Technology,Huazhong Agricultural University,Wuhan 430070,China;Institute of Crop Science,Chinese Academy of Agricultural Sciences,Beijing 100081,China;Northwest Institute of Plateau Biology,Chinese Academy of Sciences/Qinghai Provincial Key Laboratory of Crop Molecular Breeding,Xining 810008,China)

机构地区：[1]华中农业大学植物科学技术学院,武汉430070 [2]中国农业科学院作物科学研究所,北京100081 [3]中国科学院西北高原生物研究所/青海省作物分子育种重点实验室,西宁810008

出　　处：《华中农业大学学报》2019年第6期56-61,共6页Journal of Huazhong Agricultural University

基　　金：国家自然科学基金国际（地区）合作与交流项目（31861143010）;华中农业大学自主科技创新基金项目;中央高校基本科研业务费专项（2662019PY009）;青海省作物分子育种重点实验室开放课题（2017-ZJ-Y14)

摘　　要：以印度小麦品种Sujata与澳大利亚小麦品系Avocet杂交获得148个家系的重组自交系群体为材料,利用6 397对基于二代测序技术的GBS标记、705对DArT-array标记和164对SSR标记构建普通小麦高密度遗传图谱。结果显示:该图谱覆盖小麦的21条染色体,分为25个连锁群,总长度6 104.4 cM,标记间平均距离为0.84 cM。本研究构建的高密度连锁图为后续QTL定位、图位克隆和分子标记辅助选择等研究奠定基础。Genetic linkage map based on classical molecular marker platforms with low density cannot meet the requirements for wheat functional genomics studies due to its huge and complex genome.148 recombinant inbred lines(RILs)derived from the crossing Sujata(Indian wheat variety)with Avocet(Australia wheat variety)were used to construct the high-density genetic linkage map with 6 397 genotyping-by-seqencing(GBS)markers,705 DArT-array markers and 164 simple sequence repeat(SSR)markers.21 chromosomes were covered by 25 linkage groups with a total length of 6 104.4 cM.It will provide an important resource for QTL mapping,map-based cloning and marker assisted selection in wheat breeding.

关 键 词：小麦 连锁图谱 GBS标记 DArT-array标记 SSR标记 重组自交系 

分 类 号：S512.103.2[农业科学—作物学]