1种柑橘大实蝇寄生蜂鉴定及生物学研究  

Morphological and molecular identification and biological characterization of parasitoid of Bactrocera minax

作　　者：张桂健 陈振中[1] 江楠 牛长缨[1] ZHANG Guijian;CHEN Zhenzhong;JIANG Nan;NIU Changying(College of Plant Science and Technology,Huazhong Agricultural University,Wuhan 430070,China)

机构地区：[1]华中农业大学植物科学技术学院

出　　处：《华中农业大学学报》2019年第6期98-103,共6页Journal of Huazhong Agricultural University

基　　金：国家自然科学基金项目（31661143045,31972270）;公益性行业（农业）科研专项（201503137）;国际原子能机构国际合作项目（18269)

摘　　要：介绍在我国首次发现的柑橘大实蝇寄生蜂,对其进行形态、分子鉴定及相关生物学研究。该寄生蜂胸、腹部大部分黑色,前翅脉m-cu后端开叉,触角为63~67节,整个前足橙黄色,中足胫节和跗节橙黄色,后足黑色,中胸背板有明显的盾纵沟。进一步对COI基因和28sRNA基因构建NJ进化树分析,发现该寄生蜂与全裂茧蜂属中的长尾潜蝇茧蜂Diachasmimorpha longicaudata、Diachasmimorpha kraussii、Diachasmimorpha tryoni三者亲缘关系最近。在自然条件下寄生蜂的羽化历期要明显长于柑橘大实蝇,且羽化寄生蜂的雌性比例随着温度的升高而增加。结合其形态特征,命名为柑橘大实蝇茧蜂(Diachasmimorpha feijeni)。In this paper,the parasitoid of Bactrocera minax was first recorded in China.Morphological and molecular identification and biological characterization were carried out.The results showed that the chest and the abdomen of the parasitoid of Bactrocera minax were mostly black.Vein m-cu of fore wing juse postfurcal,and the antennae were 63-67 knots.The the middle tibia and tarsus were orange-yellow,the hind leg was black,and the mesothorax had obvious“V”pattern.Results of further analyzing the NJ phylogenetic tree of COI gene and 28sRNA gene revealed that the parasitic wasp was closely related to Diachasmimorpha longicaudata,Diachasmimorpha kraussii,Diachasmimorpha tryoni.Under natural conditions,the emergence period of parasitoid was significantly longer than that of B.minax.The proportion of emerged females increased with the rise of temperatures.Combined with its morphological characteristics,it was named as Chinese citrus fly cocoon wasp(Diachasmimorpha feijeni).

关 键 词：柑橘大实蝇 寄生蜂 柑橘大实蝇茧蜂 形态与分子鉴定 生物学特性 

分 类 号：S476.3[农业科学—农业昆虫与害虫防治]