罗伊氏乳杆菌对坏死性小肠结肠炎氧化应激的保护机制  

Protective effect of Lactobacillus reuteri against oxidative stress in neonatal mice with necrotizing enterocolitis

作　　者：唐佳[1] 郭春宝[2] 龚放[1] TANG Jia;GUO Chunbao;GONG Fang(Department of Pediatrics,Yongchuan Hospital Affiliated to Chongqing Medical University,Chongqing 402160,China;Department ofHepatobiliary Surgery,Children's Hospital Affiliated to Chongqing Medical University,Chongqing 400016,China)

机构地区：[1]重庆医科大学附属永川医院新生儿科,重庆402160 [2]重庆医科大学附属儿童医院肝胆外科,重庆400014

出　　处：《南方医科大学学报》2019年第10期1221-1226,共6页Journal of Southern Medical University

基　　金：国家自然科学基金(30973440,30770950);国家自然科学基金重点项目(30330590);重庆市自然科学基金重点项目(CSTC,2008BA0021,cstc2012jjA0155)~~

摘　　要：目的探讨罗伊氏乳杆菌(L.reuteri)DSM17938菌株对坏死性小肠结肠炎(NEC)新生小鼠模型氧化应激的保护作用及可能机制。方法将96只10日龄C57BL/6J新生小鼠随机分为3组(n=32):对照组、NEC组、NEC+L.reuteri组。通过HE染色观察回盲部肠组织病理变化并行双盲病理评分。采用实时荧光定量PCR检测肠组织中TNF-α、IL-1β的基因表达水平。通过ELISA检测肠组织TNF-α、IL-1β的蛋白表达水平。采用比色法检测SOD活力及抑制率、MDA、GSH、GSSG、GSSG/GSH比值。结果与对照组相比,NEC组小鼠体质量下降(P<0.05),肠道损伤加重,病理评分增加(P<0.05),TNF-α、IL-1β在基因及蛋白水平均表达升高(P<0.05),SOD活力及抑制率、GSH降低,MDA、GSSG、GSSG/GSH比值显著升高(P<0.05)。与NEC组相比,NEC+L.reuteri组病理评分降低,TNF-α、IL-1β在基因及蛋白水平均表达降低(P<0.05),SOD活力及抑制率、GSH增加,MDA、GSSG、GSSG/GSH比值明显降低(P<0.05),两组生存率差异无统计学意义(P>0.05)。结论L.reuteri DSM17938可能通过减少肠道氧化应激、增加其抗氧化能力,从而减轻肠道炎症反应,发挥对NEC新生小鼠肠道保护作用。Objective To investigate the protective effect of L.reuteri DSM17938 strain against oxidative stress in a neonatal mouse model of necrotizing enterocolitis(NEC)and explore the possible mechanism.Methods Ninety-six 10-day-old neonatal C57BL/6J mice were equally randomized into control group,NEC group,and NEC+L.reuteri group.The pathological changes of the ileocecal intestinal tissue were evaluated with HE staining and double-blind pathological scoring.The mRNA and protein expressions of tumor necrosis factor-α(TNF-α)and interleukin-1β(IL-1β)in the intestinal tissues were detected using quantitative real-time PCR and ELISA,respectively.Colorimetric assays were used to determine the activity of superoxide dismutase(SOD)and its inhibition rate,malondialdehyde(MDA),glutathione(GSH),oxidized glutathione(GSSG),and GSSG/GSH ratio.Results Compared with those in the control group,the neonatal mice in NEC group showed significant weight loss(P<0.05),obvious intestinal injury,increased pathological scores(P<0.05),increased expressions of TNF-αand IL-1βmRNA and proteins(P<0.05),decreased SOD activity and inhibition rate,decreased GSH,and significantly increased MDA,GSSG,and GSSG/GSH ratios(P<0.05).Treatment with L.reuteri obviously decreased the pathological scores,expressions of TNF-αand IL-1β(P<0.05),MDA,GSSG,and GSSG/GSH ratio(P<0.05),and significantly increased SOD activity,its inhibition rate,and GSH level in the mice with NEC,but the survival rate was not significantly different between NEC and L.reuteri-treated groups(P>0.05).Conclusion L.reuteri DSM17938 can offer protection against NEC in mice by reducing oxidative stress and increasing antioxidant capacity of the intestinal tissue to suppress intestinal inflammations.

关 键 词：罗伊氏乳杆菌DSM17938 坏死性小肠结肠炎 炎症因子 氧化应激 

分 类 号：R28[医药卫生—中药学]