不同添加量的红豆杉叶干粉对仙居鸡BCL2和NF-κB基因表达的影响  

Effects of Various Levels of Dried Yew Leaf Powder on the Gene Expression of BCL2 and NF-κB mRNA in Xianju Chicken

作　　者：刘银兰 李国勤[1] 叶轩 李浙烽[4] 赵倩云 顾天天 田勇[1] 李昂[2] 卢立志[1] LIU Yin-lan;LI Guo-qin;YE Xuan(Institute of Animal Husbandry and Veterinary Medicine,Zhejiang Academy of Agricultural Sciences,Hangzhou,Zhejiang 310021;Fujian Agriculture and Forestry University,Fuzhou,Fujian 350000;Zhejiang Xianju Breeding Farm,Xianju,Zhejiang 311719)

机构地区：[1]浙江省农业科学院畜牧兽医研究所,浙江杭州310021 [2]福建农林大学,福建福州350000 [3]浙江省仙居种鸡场,浙江仙居311719 [4]杭州康德权饲料有限公司,浙江杭州311107

出　　处：《安徽农业科学》2019年第21期92-95,共4页Journal of Anhui Agricultural Sciences

基　　金：国家农业科技成果转化项目(2014GB2C200207);浙江省农业科学院仙居鸡产业团队科技特派员项目(103);仙居县科技项目(2018B04)

摘　　要：[目的]研究在饲粮中添加不同水平的红豆杉叶干粉对仙居鸡肝脏、脾脏、肺脏、肾脏、十二指肠、胸肌和腿肌中BCL2和NF-κB mRNA表达量的影响,以确定红豆杉叶干粉在仙居鸡饲粮中适宜的添加量,并探讨红豆杉叶干粉作为功能性饲料添加剂用于仙居鸡生产的可行性。[方法]试验选取140日龄健康生长状况良好、体重相近的仙居鸡400只作为试验鸡,将其随机分为4组,A组为对照组,饲喂基础饲粮;B、C和D组为试验组,在基础日粮中分别添加2%、3%、4%的红豆杉叶干粉,试验期90 d。[结果]与A组相比,C、D组鸡脾脏BCL2基因表达量均显著降低(P<0.05);B组鸡肾脏BCL2表达量显著升高;各组鸡十二指肠BCL2表达量均显著降低(P<0.05);C、D组鸡胸肌BCL2表达量显著升高(P<0.05),而B组鸡胸肌BCL2表达量显著降低(P<0.05);D组鸡腿肌BCL2表达量显著升高(P<0.05),B组腿肌BCL2表达量显著降低(P<0.05)。与A组相比,B、D组鸡肝脏NF-κB基因表达量显著升高(P<0.05);B、C组肺脏NF-κB基因表达量显著降低(P<0.05);D组脾NF-κB基因表达量显著降低(P<0.05);各试验组十二指肠NF-κB基因表达量显著降低(P<0.05),B、C组鸡胸肌NF-κB基因表达量显著降低(P<0.05),C组腿肌NF-κB基因表达量显著降低(P<0.05)。[结论]不同添加量红豆杉叶干粉对仙居鸡BCL2和NF-κB基因组织表达水平具有一定程度的影响。[Objective]To study the effects of different levels of dried yew leaf powder on the expression amount of BCL2 and NF-κB mRNA in the liver,spleen,lung,kidney,intestine,chest muscle and leg muscle of Xianju chicken and determine the appropriate addition level of dried yew leaf powder in the diet of Xianju chicken,and explore the feasibility of using dried yew leaf powder as a functional feed additive for the production of Xianju chicken.[Method]A total of 400 140-day-old healthy Xianju chicken with good growth status and similar body weight were randomly divided into four groups.The chicken in group A(control group)were fed the basic diet.The chicken in groups B,C and D(experimental groups)were fed with the basal diet with adding 2%,3%and 4%dried yew leaf powder respectively.The test period lasted for 90 days.[Result]Compared with group A,the expression levels of BCL2 gene in chicken spleen of group C and D were significantly decreased(P<0.05).The expression of BCL2 in group B was significantly increased(P<0.05).The expression level of BCL2 in chicken duodenum of three test groups was significantly decreased(P<0.05).The expression level of BCL2 in breast muscle of chicken in group C and D were significantly increased(P<0.05),and that of group B was significantly decreased(P<0.05).The expression of BCL2 in leg muscle of chicken in group D was significantly increased(P<0.05),but that in group C was significantly decreased(P<0.05).Compared with group A,the expression level of NF-κB gene in chicken liver of groups B and D were significantly increased(P<0.05).The expression level of NF-κB gene in chicken lungs of groups B and C were significantly decreased(P<0.05).The expression level of NF-κB gene in chicken spleen of group D was significantly decreased(P<0.05).The expression level of NF-κB gene in chicken duodenum of every test group was significantly decreased(P<0.05).The expression level of NF-κB gene in chicken chest muscles of groups B and C were significantly decreased(P<0.05),but the expression level of NF

关 键 词：红豆杉叶干粉 仙居鸡 BCL2 NF-ΚB 

分 类 号：S831.4[农业科学—畜牧学]