基于SRAP分子标记的铁皮石斛遗传多样性分析  

Genetic diversity analysis of Dendrobium officinale based on SRAP molecular markers

作　　者：杨贞 蔡友铭[1] 张永春[1] 杨柳燕[1] 王艺程[1] YANG Zhen;CAI You-ming;ZHANG Yong-chun;YANG Liu-yan;WANG Yi-cheng(Forest&Fruit Tree Research Institute,Shanghai Academy of Agricultural Sciences,Shanghai Key Laboratory of Protected Horticultural Technology,Shanghai 201403,China)

机构地区：[1]上海市农业科学院林木果树研究所上海市设施园艺技术重点实验室

出　　处：《上海农业学报》2019年第5期23-27,共5页Acta Agriculturae Shanghai

基　　金：上海市农委攻关项目[沪农科攻字(2015)第4-11号];上海市农委产业体系建设专项[沪农科产字(2017)第8号];上海市农委成果转化项目[沪农科转字(2014)第2号];上海市绿化和市容管理局科技攻关项目(G171207);上海市科委研发平台专项(15DZ2291000)

摘　　要：利用SRAP分子标记法对13份来自中国不同产地的铁皮石斛品种的基因组DNA进行分析,从分子水平研究铁皮石斛的遗传多样性和亲缘关系。结果表明:从81对引物中筛选出15对引物,在13份材料中共扩增出238条清晰的多态性条带,占总条带数的96.75%。13份材料间的遗传相似系数为0.44—0.79,在相似系数0.46处13份材料被划分为3个类群。研究结果可为不同产地铁皮石斛品种亲缘关系的鉴定以及种质资源的利用与保护提供理论依据。The genomic DNA of 13 Dendrobium officinale varieties from different regions of China was analyzed by SRAP molecular marker method.The genetic diversity and genetic relationship of D.officinale were studied at molecular level.The results showed that 15 pairs of primers were screened out from 81 pairs of primers and 238 clear polymorphic bands were amplified in 13 materials,accounting for 96.75%of the total bands.The genetic similarity coefficients of 13 materials ranged from 0.44 to 0.79,and 13 materials were divided into three groups at 0.46.The results could provide theoretical basis for the identification of genetic relationship among D.officinale varieties from different regions and the utilization and protection of germplasm resources.

关 键 词：铁皮石斛 亲缘关系 SRAP 聚类分析 遗传多样性 

分 类 号：S567.2[农业科学—中草药栽培]