Cr(VI)还原微生物筛选、分离及鉴定  

Screening,Isolation And Identification of Microorganisms With Chromium Tolerance

作　　者：王思雅 左群 刘朝阳 皮海廷 李宁[1] Wang Siya;Zuo Qun;Liu Chaoyang;Pi Haiting;Li Ning(School of water conservancy and environment,Hubei Yichang 443002;College of biology and pharmacy,three gorges university,Hubei Yichang 443002)

机构地区：[1]三峡大学水利与环境学院,湖北宜昌443002 [2]三峡大学生物与制药学院,湖北宜昌443002

出　　处：《科技风》2019年第32期232-233,共2页

摘　　要：[目标]本文以分离并获得具有Cr(VI)耐受、还原能力的微生物为目标,[方法]从受污染土壤提取液中富集Cr(Ⅵ)耐受菌株,并对其进行分离、鉴定。[结果]实验共筛选出四株具有Cr(VI)耐受、还原菌株,其中2株菌株的Cr(VI)耐受能力较好,W1菌株和W3菌株。这两株菌株均能在铬浓度为100mg/L的培养液中生存,且具有一定的还原Cr的能力。[意义]这两株菌株的筛选、分离与鉴定丰富了处理铬污染微生物的生物资料,对微生物处理重金属污染具有重大意义。This paper separation,screening and identification of microorganisms capable of strong resistance to chromium target.By the extract of contaminated soil Cr(Ⅵ)strains screened tolerance,and carries on the separation,Enrichment and detection of PCR.[results]a total of four tolerant strains were screened out in the experiment,but two of them were in poor growth condition,so only the W1 strains and W3 strains with good growth condition were retained.These two strains can survive in the culture solution with chromium concentration of 100mg/L.[Significance]The screening,isolation and identification of these two strains greatly enriched the biological data for treating chromium contaminated microorganisms,which was of great significance for microbial treatment of heavy metal pollution.

关 键 词：铬污染 耐受菌 筛选 鉴定 

分 类 号：TS2[轻工技术与工程—食品科学与工程]