利拉鲁肽和有氧运动对肥胖大鼠下丘脑弓状核神经元凋亡的影响（英文）  

Effect of liraglutide and aerobic exercise on apoptosis of arcuate nucleus neurons in obese rats

作　　者：杨威[1] 赵胜男 荣曦[1] 刘红[1] Wei Yang;Shengnan Zhao;Xi Rong;Hong Liu(Department of Geriatrics Endocrinology and Metabolism,The First Affiliated Hospital of Guangxi Medical University,Nanning 530021,Guangxi,China)

机构地区：[1]广西医科大学第一附属医院老年内分泌科

出　　处：《广西医科大学学报》2019年第10期1549-1554,共6页Journal of Guangxi Medical University

基　　金：supported by grants from the National Natural Science Foundation of China(No.81760154;No.31600677);Guangxi Natural Science Foundation(No.2017JJA10168)

摘　　要：目的:探讨利拉鲁肽和有氧运动对肥胖大鼠下丘脑弓状核(ARC)神经元形态学及细胞凋亡的影响。方法:高脂饮食建立肥胖大鼠模型,并随机分为高脂饮食+生理盐水组(HS组)、高脂饮食+利拉鲁肽组(HL组)、高脂饮食+有氧运动组(HE组);基础饲料喂养作为对照组(NC组)。HL组予皮下注射利拉鲁肽200μg/(kg·d),NC组和HS组均予皮下注射等量生理盐水,HE组进行无负重游泳训练,60 min/d。干预6周后,采用尼式染色观察下丘脑ARC神经元形态结构,免疫荧光染色法检测Bax、Bcl-2表达水平,TUNEL法检测神经细胞凋亡率。结果:尼式染色结果显示,NC组下丘脑弓状核区神经元结构完整,排列紧密;HS组下丘脑ARC神经元显著减少,排列紊乱,可见核固缩等明显改变;HL组和HE组神经细胞均有改善。与NC组相比,HS组细胞凋亡率和Bax表达显著增高,Bcl-2表达显著降低(均P<0.01)。利拉鲁肽或有氧运动可降低TUNEL阳性细胞数和Bax表达,同时上调Bcl-2表达(均P<0.01),且HL组改善效果更明显。结论:利拉鲁肽可明显改善下丘脑ARC神经元形态结构,抑制肥胖大鼠神经元凋亡,且效果优于有氧运动。Objective:To investigate the effects of liraglutide and aerobic exercise on morphology and apoptosis of arcuate nucleus(ARC)neurons in obese rats.Methods:Obese rats were established by a high-fat diet and randomly divided into high-fat diet+saline(HS)group,high-fat diet+liraglutide(HL)group,and high-fat diet+aerobic exercise(HE)group.Untreated healthy rats served as the normal control(NC)group.The HL group rats received subcutaneous injection of liraglutide 200μg/(kg·d).The NC group and HS group rats were given subcutaneous injection of the same volume of normal saline.Rats in the HE group were subjected to weightless swimming training for 60 min once day.After 6 weeks of intervention,the morphological structure of neurons in ARC of hypothalamus was observed by Nissl staining.The expression levels of Bax and Bcl-2 were determined by immunofluorescence.The apoptotic rate was detected by terminal deoxynucleotidyl transferase d UTP nick end labeling(TUNEL)staining.Results:Compared with the NC group,ARC neurons were occasionally pyknotic and decreased in number in the hippocampus of the HS rats,and exhibited disordered arrangement,while milder neuronal injury and more cells were observed in the rats of the HL group and HE group.Compared with the HS group,liraglutide treatment or aerobic exercise decreased the number of TUNEL-positive cells and the expression of Bax concomitantly with upregulated Bcl-2(P<0.01).The changes were more marked in the HL group.Conclusion:Liraglutide could alleviate neuronal damages of the ARC by inhibiting apoptosis in obese rats,and its effect was superior to that of aerobic exercise.

关 键 词：利拉鲁肽 有氧运动 肥胖大鼠 下丘脑 细胞凋亡 

分 类 号：R338[医药卫生—人体生理学]