羊膜泪小管支架治疗去势雄兔干眼症的实验研究  

Experimental study of amniotic lacrimal stent in the treatment of dry eye in castrated male rabbits

作　　者：黎彪 袁晴[1] 朱佩文[1] 林启 闵幼兰 石文卿 叶蕾[1] 邵毅[1] Biao Li;Qing Yuan;Pei-Wen Zhu;Qi Lin;You-Lan Min;Wen-Qing Shi;Lei Ye;Yi Shao(Department of Ophthalmology,the First Affiliated Hospital of Nanchang University,Nanchang 330006,Jiangxi Province,China)

机构地区：[1]南昌大学第一附属医院眼科

出　　处：《国际眼科杂志》2019年第11期1834-1839,共6页International Eye Science

基　　金：国家自然科学基金资助项目(No.81660158,81460092,81400372);江西省重点研发项目重大项目(No.20151BBG70223);江西省青年科学基金项目(No.20151BAB215016,20161ACB21017);江西省教育厅重点项目(No.GJJ160020);江西省卫计委科技计划面上项目(No.20175116)~~

摘　　要：目的:探究羊膜泪小管支架对手术阉割雄兔干眼症的影响。方法:将36只成年雄性兔分组,其中A组行假阉割手术,B组假植入泪小管支架,C组植入泪小管支架。分别于植入前和植入后2、4、6wk行角膜荧光素染色(FL)和泪液分泌试验(SⅠt)检查,共聚焦显微镜观察角膜神经变化。结果:A组角膜光滑、清晰,FL染色不明显;B组角膜粗糙,角膜上皮见点染色斑点;C组角膜较假手术组更为光滑、清晰,无明显点状染色。各组植入后2、4、6wk时FL染色评分和SⅠt比较,差异有统计学意义(均P<0.05)。共聚焦显微镜下可见A组为相对直的神经丛,量多,清晰可见;B组角膜上皮细胞下神经可见弯曲,数量有所减少;C组角膜上皮下神经相对直,与正常雄兔角膜上皮下神经相比,数量略有减少。结论:羊膜泪小管支架可显著改善阉割去势雄兔干眼症的症状和体征,具有治疗作用。AIM:To explore the effect of amniotic lacrimal stent graft on castrated male rabbits of dry eye.METHODS:Thirty-six adult male rabbits were divided into two groups.Group A was treated with false castration,group B was pseudo-implantation group,and group C was implanted with lacrimal canal.Fluorescein(FL)staining and scoring and SⅠt examination,and confocal microscopy were performed at 2,4,6wk after implantation.RESULTS:The cornea of group A was smooth and clear,and the FL staining was not obvious.In group B,the cornea was rough,and the corneal epithelium was spotted with stained spots.The corneal surface of group C was smoother and more transparent than that of sham operation group and the punctate staining was not obviously.The FL staining scores and SⅠt examination were significant(P<0.05)at 2,4,and 6wk after implantation.FL staining and scoring and SⅠt,tear protein determination,confocal microscopy changes in corneal nerve.Confocal microscopy showed that group A was a relatively straight plexus,the amount was large,clearly visible.In the group B,the inferior nerve of the corneal epithelial cells was visible and the number was reduced.The corneal epithelial nerve in group C was relatively straight,and the number was slightly reduced compared with the normal male rabbit corneal epithelial nerve.CONCLUSION:The amniotic lacrimal duct stent can significantly improve the symptoms and signs of castrated castrated male rabbit dry eye syndrome,and has a therapeutic effect.

关 键 词：羊膜 泪小管支架 干眼 阉割去势雄兔 治疗 

分 类 号：S85[农业科学—兽医学]