消暑促情散缓解雌鼠热应激引起的脏器损伤研究  

Xiaoshucuqing Powder Ameliorates Heat Stress Induced Organ Damage in Female Mice

作　　者：李金灿 田晓刚 宫新城 LI Jin-can;TIAN Xiao-gang;GONG Xin-cheng(College of Traditional Chinese Veterinary Medicine,Agricultural University of Hebei,Baoding,Hebei,071001,China)

机构地区：[1]河北农业大学动物医学院中兽医学院

出　　处：《动物医学进展》2019年第11期50-54,共5页Progress In Veterinary Medicine

基　　金：河北省科技计划项目(16236605D-5)

摘　　要：为探讨消暑促情散缓解热应激对内脏器官的影响,将60只成年昆明雌鼠随机分成3组,室温对照组(25℃,基础日粮)、热应激对照组(33℃,基础日粮)和中药组(33℃,基础日粮+1%消暑促情散)。试验期间测定小鼠各项生长指标。30 d后脱颈处死,称量每只小鼠体重以及肝、脾、肺、肾的重量,而后将组织放入波恩氏液中固定,HE染色并观察内脏组织结构变化。结果表明,中药组的增重率和生长率分别为80.23%和1.96%,均显著高于热应激对照组(P<0.05);中药组的料肉比为27.83,低于热应激对照组;与热应激对照组相比,中药组的小鼠内脏指数均有所升高,其中肾脏指数显著升高(P<0.05),脾脏以及肺脏指数极显著升高(P<0.01);通过切片可以看出,热应激对照组肝细胞出现脂肪变性,细胞核浓染以及裂解消失,脾脏炎性细胞明显增多,肺脏组织中肺泡收缩,且有大量炎性细胞浸润,肾脏有明显出血,而中药组有显著改善。说明消暑促情散能够明显缓解热应激造成小鼠生长不良及内脏的损伤。The present study was conducted to investigate the protective effect of Xiaoshucuqing powder on organs damage caused by heat stress.Sixty six-week-old female Kunming mice were randomly divided into 3 groups,i.e.,the room temperature control group(25℃,basal diet),the heat stress control group(33℃,basal diet)and the herbal medicine group(33℃,basal diet supplemented with 1%Xiaoshucuqing powder).During the trial,the growth indexes of mice were determined.These mice were sacrificed by cervical dislocation after blood sampling at Day 30.Intact liver,spleen,lung and kidney were carefully collected and weighed.The organ samples were fixed in Bouin s solution and embedded in paraffin for histological assays and haematoxylin-eosin(HE)staining to observe the changes of organ architecture.The results showed that the weight gain and growth rate in the herbal group were 80.23%and 1.96%,respectively,which were significantly increased compared to that in the heat stress control group(P<0.05).The feed conversion ratio in the herbal medicine group was 27.83,which was obviously decreased compared to that in heat stress control group.Compared with the heat stress control group,the organ indexes of mice in the herbal medicine group increased.Renal index was significantly increased(P<0.05)and spleen and lung indices were significantly increased(P<0.01).HE staining showed that in the heat stress control group,light fatty degeneration was observed in hepatocytes,hyperchromatic nuclei and disappeared cleavage were also observed.Other observations includes infiltration of inflammatory cells in spleen,the alveolar contraction in the lung tissue with large number of inflammatory cells infiltration,and obvious bleeding in the kidney.All the above mentioned pathological changes were significantly alleviated in the herbal medicine group.The results indicated that the Xiaoshucuqing powder can obviously alleviate the poor growth and organ damage caused by heat stress in mice.

关 键 词：消暑促情散 热应激 小鼠 脏器损伤 

分 类 号：S853.7[农业科学—临床兽医学]