鸡球虫体外培养模型研究进展  

Progress on Culture Models of Chicken Coccidia in Vitro

作　　者：吴彩艳 戚南山 李娟 吕敏娜 林栩慧 胡俊菁 于林增 廖申权 孙铭飞 WU Cai-yan;QI Nan-shan;LI Juan;LV Min-na;LIN Xu-hui;Hu Jun-jing;Yu Lin-zeng;LIAO Shen-quan;SUN Ming-fei(Institute of Animal Health,Guangdong Academy of Agricultural Sciences,Scientific Observation and Experiment Station of Veterinary Drugs and Diagnostic Techniques of Guangdong Province,Ministry of Agriculture and Rural Affairs P.R.China,Key Laboratory of Livestock Disease Prevention of Guangdong Province,Guangzhou,510640,China)

机构地区：[1]广东省农业科学院动物卫生研究所农业农村部兽用药物与诊断技术广东科学观测实验站广东省畜禽疫病防治研究重点实验室

出　　处：《动物医学进展》2019年第11期91-95,共5页Progress In Veterinary Medicine

基　　金：国家重点研发计划专项(2016YFD0501303);国家自然科学基金项目(31402186);广东省科技计划项目(2017A020225025);国家星火计划项目(2015GA780050)

摘　　要：鸡球虫病对养鸡业危害十分严重,由于抗球虫药物的使用甚至滥用,鸡球虫的抗药性日趋广泛而严峻。为了研究鸡球虫入侵机制以及快速高通量筛选抗球虫制剂的需要,鸡球虫的体外培养技术从20世纪70年代初至今已历经近50年,研究取得了一定的进展,逐步形成了一整套比较完备的体外培养技术体系。论文主要就鸡球虫的原代细胞培养模型、传代细胞培养模型和鸡胚培养模型在建立和应用方面进行详细阐述,为研究鸡球虫相关工作提供参考。Coccidiosis is very harmful to poultry industry.The resistance of chicken coccidia is becoming more and more serious with the use and abuse of coccidial drugs.In order to study the invasion mechanism of coccidia and the need for rapid screening of anti coccidial drugs,the culture technology of coccidia in vitro has lasted for nearly 50 years since the beginning of 1970s.The research has made some progress and gradually formed a complete set of more complete culture system in vitro.In this paper,we detailed the primary cell culture model,cell culture model and chicken embryo culture model of coccidia in establishment and application,in order to provide a technical platform for studying coccidia.

关 键 词：鸡球虫 体外 培养模型 

分 类 号：S852.71[农业科学—基础兽医学]