山楂叶总黄酮调控肾组织p38MAPK信号通路干预糖尿病肾病大鼠氧化应激损伤的实验研究  

Experimental study of hawthorn leaves flavonoids for intervening oxidative stress injury by regulating renal tissue p38MAPK signal pathway in diabetic nephropathy rats

作　　者：覃春美 李刚 程宗华[1] 王茜茜[1] 邹懿[1] QIN Chunmei;LI Gang;CHENG Zonghua;WANG Qianqian;ZOU Yi(Department of Nephrology,Luzhou Municipal People′s Hospital,Luzhou,Sichuan 646000,China;Department of Oncology,Luzhou Municipal People′s Hospital,Luzhou,Sichuan 646000,China)

机构地区：[1]四川省泸州市人民医院肾病内科,四川泸州646000 [2]四川省泸州市人民医院肿瘤科,四川泸州646000

出　　处：《检验医学与临床》2019年第21期3131-3134,共4页Laboratory Medicine and Clinic

摘　　要：目的探讨山楂叶总黄酮调控糖尿病肾病大鼠肾组织氧化应激损伤的作用和机制。方法35只SD雄性大鼠随机分为正常对照组和模型组,模型组给予高脂饮食及链脲佐菌素(STZ)腹腔注射建立糖尿病大鼠模型。成模的大鼠随机分为糖尿病肾病组、厄贝沙坦组、山楂叶总黄酮组,分别于实验第4、8、12周测体质量、24 h尿蛋白定量,12周后处死大鼠,留取血标本分别检测尿素氮、肌酐、三酰甘油、一氧化氮、丙二醛和超氧化物歧化酶水平,HE及Masson染色观察肾脏病理改变,免疫组化检测肾组织p38MAPK、p-p38MAPK蛋白的表达。结果山楂叶总黄酮能改善糖尿病肾病模型鼠一般情况、体质量,减少尿蛋白,与糖尿病肾病组相比,山楂叶总黄酮组大鼠肾组织病理损害明显减轻,血清尿素氮、肌酐、三酰甘油等指标明显改善(P<0.05),一氧化氮、超氧化物歧化酶水平增加(P<0.05),同时丙二醛水平显著降低(P<0.05),肾组织p38MAPK、p-p38MAPK蛋白水平下调(P<0.05)。结论山楂叶总黄酮可能通过抑制氧化应激对糖尿病肾病进展起积极作用,并可能通过调控糖尿病肾病肾组织p38MAPK信号转导通路,改善氧化应激对肾组织的损伤。Objective To explore the effects and mechanism of hawthorn leaves flavonoids(HLF)for regulating the oxidative stress injury in renal tissue of diabetic nephropathy rats.Methods Thirty-five male SD rats were randomly divided into the normal control group(CON group)and model group.The model group was given the high fat diet and streptozotocin(STZ)by abdominal cavity injection for establishing the diabetic rat model.The modeled rats were randomly divided into the diabetic kidney disease(DKD)group,irbesartan(IRB)group and HLF group.The body weight and 24 h urinary protein levels were measured at 4,8 and 12 weeks of experiment respectively.The rats were sacrificed after 12 weeks.The blood samples were taken to detect the levels of urea nitrogen,creatinine,triglyceride,nitric oxide,malondialdehyde and superoxide dismutase.The renal pathologic changes were observed by HE and Masson staining.Immunohistochemistry was applied to detect the protein expression of p38MAPK and p-p38MAPK.Results HLF could improve the general condition and body weight,and reduced urinary protein in the diabetic nephropathy model rats.Compared with the diabetic nephropathy group,the pathological damages of renal tissue in the HLF group were significantly alleviated,the indicators of serum urea nitrogen,creatinine,triglyceride,etc.were significantly improved(P<0.05),while the levels of nitric oxide and superoxide dismutase were increased(P<0.05),meanwhile the malondialdehyde level was significantly decreased(P<0.05),and the levels of renal tissue p38MAPK and p-p38MAPK protein were down-regulated(P<0.05).Conclusion HLF may play an active role in the progression of diabetic nephropathy by inhibiting oxidative stress,and improve the injury of oxidative stress on renal tissue by regulating renal tissue p38MAPK signal transduction pathway in diabetic nephrapathy.

关 键 词：山楂叶总黄酮 糖尿病肾病 P38MAPK 氧化应激 

分 类 号：R446.69[医药卫生—诊断学]