小麦粒重相关基因TaCYP78A5功能标记开发及验证  

Development and validation of the functional marker of grain weight-related gene TaCYP78A5 in wheat(Triticum aestivum L.)

作　　者：司文洁 吴林楠 郭利建 周梦蝶 刘香利[1,2] 马猛 赵惠贤[1,2] SI Wen-Jie;WU Lin-Nan;GUO Li-Jian;ZHOU Meng-Die;LIU Xiang-Li;MA Meng;ZHAO Hui-Xian(College of Life Science,Northwest A&F University,Yangling 712100,Shaanxi,China;State Key Laboratory of Crop Stress Biology for Arid Areas/Northwest A&F University,Yangling 712100,Shaanxi,China)

机构地区：[1]西北农林科技大学生命科学学院,陕西杨陵712100 [2]西北农林科技大学/旱区作物逆境生物学国家重点实验室,陕西杨凌712100

出　　处：《作物学报》2019年第12期1905-1911,共7页Acta Agronomica Sinica

基　　金：陕西杨凌示范区产学研用协同创新重大项目计划(2017CXY-01);国家自然科学基金项目(31701419,31471482)资助~~

摘　　要：为了开发小麦粒重相关基因TaCYP78A5(Triticum aestivum Cytochrome P450 78A5)的功能标记,挖掘与千粒重性状相关的优异等位变异,本研究通过对30份不同品种小麦TaCYP78A5启动子区测序及比对鉴定,并根据SNP位点差异开发TaCYP78A5-2A启动子区功能标记CAPS-5Ap。结果表明,在30份不同小麦品种中TaCYP78A5-2A启动子区域出现5个SNP位点差异,可将30份不同品种小麦分为TaCYP78A5-2Ap-HapI和TaCYP78A5-2Ap-HapII两种单倍型;以323份现代育成小麦品种验证发现,TaCYP78A5-2Ap-HapI的分布频率为17.96%,TaCYP78A5-2Ap-HapII的分布频率为82.04%,表明CAPS-5Ap标记可用于小麦TaCYP78A5-2A启动子序列2种单倍型的鉴定。此外,关联分析发现,CAPS-5Ap标记与粒重相关,且TaCYP78A5-2Ap-HapII是提高千粒重的优异单倍型。研究结果为小麦分子标记辅助选择和性状改良提供理论依据。In order to develop the functional marker of wheat grain weight related gene TaCYP78A5(Triticum aestivum Cytochrome P450 78A5)and excavate superior alleles for 1000-grain weight,functional marker CAPS-5Ap was developed according to the different SNP loci of TaCYP78A5-2A by analyzing the TaCYP78A5 promoter sequence of 30 wheat varieties and alignment evaluation.There were five SNP loci in TaCYP78A5-2A promoter regions of 30 wheat varieties,which could be divided into two kinds of genotype,TaCYP78A5-2Ap-HapIand TaCYP78A5-2Ap-HapII.The frequency of TaCYP78A5-2Ap-HapI and TaCYP78A5-2Ap-HapII in 323 modern varieties of wheat was 17.96%and 82.04%respectively,which indicating that the CAPS-5Ap can be used to identify two genotypes of TaCYP78A5-2A promoter sequence in wheat.What's more,the correlation between the genotype data and 1000-grain weight phenotype data of 323 modern varieties was analyzed,showing that CAPS-5Ap marker associated with grain weight,and TaCYP78A5-2Ap-HapII was the superior genotype for 1000-grain weight.These results may provide useful information for molecular marker assisted selection and character improvement in breeding for high yield wheat.

关 键 词：小麦 粒重 TaCYP78A5-2A SNP 功能标记 

分 类 号：S51[农业科学—作物学]