桑黄粗多糖对被动吸烟模型小鼠运动能力和骨骼肌自由基代谢的影响  

Effects of phellinus igniarius crude polysaccharides on sporting ability and free radical metabolism of skeletal muscle in mice suffering passive smoking

作　　者：万华喆[1] 柴广新 肖晓玲[3] 黄文英[4] Wan Huazhe;Chai Guangxin;Xiao Xiaoling;Huang Wenying(Science and Technology College of Nanchang Hangkong University,Nanchang 332020,Jiangxi Province,China;Physical Education and Health School of East China Normal University,Shanghai 200241,China;Jiangxi University of Science and Technology,Nanchang 330200,Jiangxi Province,China;Sports College of Jiangxi Normal University,Nanchang 330022,Jiangxi Province,China)

机构地区：[1]南昌航空大学科技学院,江西省南昌市332020 [2]华东师范大学体育与健康学院,上海市200241 [3]江西科技职业学院,江西省南昌市330200 [4]江西师范大学体育学院,江西省南昌市330022

出　　处：《中国组织工程研究》2020年第5期689-693,共5页Chinese Journal of Tissue Engineering Research

基　　金：江西省教育厅科学技术研究项目(GJJ161595)，项目负责人：万华喆~~

摘　　要：背景:香烟烟雾中含有大量多种类型氧自由基和细胞毒性成分,被动吸烟会损害呼吸、心血管系统功能,导致骨骼肌氧化损伤和运动能力下降。目的:观察具有抗氧化应激作用的桑黄粗多糖对被动吸烟模型小鼠运动能力和骨骼肌氧化应激的影响,为被动吸烟小鼠骨骼肌过氧化损伤和运动能力下降的防治提供思路。方法:将21只雄性昆明小鼠随机分为3组:桑黄组采用桑黄粗多糖灌胃然后被动吸烟,对照组采用蒸馏水灌胃然后被动吸烟,空白组单纯蒸馏水灌胃。各组干预持续4周,然后进行1次力竭游泳运动,记录各组小鼠游泳力竭时间。运动结束后检测小鼠双侧腓肠肌超氧化物歧化酶、过氧化氢酶、谷胱甘肽还原酶与Ca^2+-Mg^2+-ATP、Na^+-K^+-ATP酶活性及丙二醛含量。结果与结论:(1)与空白组相比,对照组小鼠力竭游泳时间缩短(P<0.01);与对照组相比,桑黄组小鼠力竭游泳时间延长(P<0.01);(2)与空白组相比,对照组小鼠超氧化物歧化酶、谷胱甘肽还原酶、过氧化氢酶活性和Ca^2+-Mg^2+-ATP、Na+-K+-ATP酶活性显著降低(P<0.01),丙二醛含量显著升高(P<0.01);(3)与对照组相比,桑黄组小鼠超氧化物歧化酶、谷胱甘肽还原酶、过氧化氢酶活性和Ca^2+-Mg^2+-ATP、Na^+-K^+-ATP活性升高(P<0.05,P<0.01),丙二醛含量显著降低(P<0.01);(4)相关性分析显示:力竭游泳时间与谷胱甘肽还原酶、过氧化氢酶、超氧化物歧化酶活性呈正相关(r>0.6,P<0.05),与丙二醛呈负相关(r=-0.637,P<0.05);Ca^2+-Mg^2+-ATP、Na^+-K^+-ATP酶活性与谷胱甘肽还原酶、过氧化氢酶及超氧化物歧化酶活性呈正相关(r> 0.6,P<0.05),与丙二醛呈负相关(r<-0.6,P<0.05);(5)结果提示:桑黄粗多糖可以提高被动吸烟模型小鼠骨骼肌抗氧化酶活性,减轻脂质过氧化反应,提高运动能力。实验方案经江西师范大学体育综合实验中心实验室伦理委员会批准(批准号:201703)。BACKGROUND:Cigarette smoke contains a large number of types of oxygen free radicals and cytotoxic components.Passive smoking will impair respiratory and cardiovascular system functions,and result in oxidative damage of skeletal muscle and decreased exercise ability.OBJECTIVE:To investigate the effect of phellinus igniarius crude polysaccharides on the exercise capacity and free radical metabolism of skeletal muscle in mice suffering passive smoking,so as to provide ideas for the prevention and treatment of peroxidative damage of skeletal muscle and depression of exercise capacity in rats suffering passive smoking.METHODS:Twenty-one male Kunming mice were randomly assigned to three groups:gavage with phellinus igniarius crude polysaccharides and suffering passive smoking(phellinus group),gavage with distilled water and suffering passive smoking(control group),and only gavage with distilled water(blank group).After 4 consecutive weeks,the mice were forced to take an exhausted swimming,and sacrificed subsequently.Exhausted swimming time was recorded.The bilateral gastrocnemius muscle tissues were obtained,in which the vitality of superoxide dismutase,catalase,glutathion reductase and Ca2+-Mg2+-ATP and Na+-K+-ATP activity,and the concentration of malonaldehyde were measured.RESULTS AND CONCLUSION:(1) The swimming time of mice in the control group was shorter than that in the blank group(P<0.01).The swimming time of mice in the phellinus group was longer than that in the control group(P<0.01).(2) Compared with the blank group,the vitality of superoxide dismutase,catalase,glutathion reductase and Ca^2+-Mg^2+-ATP and Na^+-K^+-ATP activity in the control group were decreased(P<0.01),and the concentration of malonaldehyde was increased(P<0.01).(3) When compared with the control group,the vitality of those metabolic index in the phellinus group were increased(P<0.05,P<0.01),and the concentration of malonaldehyde was decreased(P<0.01).(4) Correlation analysis showed that exhausted swimming time was positively correlated wit

关 键 词：桑黄粗多糖 灌胃 小鼠 被动吸烟 氧化应激 脂质过氧化反应 抗氧化酶 骨骼肌 运动能力 相关性分析 

分 类 号：R453[医药卫生—治疗学] R875[医药卫生—临床医学]