不同介质对脂肪干细胞活性短期保存的影响  

Effects of different storage media on the activity of human adipose-derived stem cell

作　　者：吴引弟 刘宏伟[1] Wu Yindi;Liu Hongwei(Department of Plastic Surgery,the First Affiliated Hospital of Jinan University,Guangzhou 510632,China)

机构地区：[1]暨南大学附属第一医院整形外科,广州510632

出　　处：《中华医学美学美容杂志》2019年第5期388-391,共4页Chinese Journal of Medical Aesthetics and Cosmetology

基　　金：国家自然科学基金(81272100,81372065);广州市科技计划资助(201300000091,201508020253)。

摘　　要：目的探讨10%富血小板血浆(PRP)、10%人血清、生理盐水对脂肪干细胞(ADSC)活性的影响,确定其适宜的保存环境。方法分离培养ADSC,将ADSC分别保存于10%PRP、10%人血清、生理盐水中,在室温下放置2 h,分别检测细胞的增殖能力、成骨分化能力和成活率,确定ADSC的适宜保存介质。结果与生理盐水组比较,ADSC在10%PRP、10%人血清中表现出较高的增殖能力和分化能力,且这两组细胞成活率均较生理盐水组高,其中10%人血清组细胞成活率最高,能够达到78.08%,其次为10%PRP组,为75.68%。生理盐水组成活情况最差,为62.34%。结论10%人血清最适合ADSC的短期保存。Objective To determine the proper storage environment by comparing the activity of adipose-derived stem cell(ADSC)at 10%PRP,10%HS and 0.9%NaCl.Methods ADSC was isolated and cultured.Then the ADSC was stored in 10%PRP,10%HS,and 0.9%Nacl at room temperature(RT,about 26℃)for 2 hours.After that the proliferation ability,survival rate and differentiation ability of the cells were detected.Finally storage media of ADSC was determined.Results Compared with 0.9%NaCl,ADSC showed a better proliferation and higher differentiation ability at 10%PRP and 10%HS;besides,the survival rates of two groups were higher than the group of 0.9%Nacl.Among these groups,the survive rate of 10%HS and 10%PRP were 78.08%and 75.68%respectively,while it was 62.34%in 0.9%NaCl group.Conclusions 10%HS is more suitable for short-term preservation of ADSC.

关 键 词：乳房美容 干细胞 保存 生物学 血清 富含血小板血浆 脂肪来源间充质干细胞 

分 类 号：R32[医药卫生—人体解剖和组织胚胎学]