Chemerin对卵巢颗粒细胞的影响及对PCOS发病机制的研究  

Effect of Chemerin on ovarian granulosa cells and pathogenesis of polycystic ovary syndrome

作　　者：蔡留芸 胡建国[1] 龚阳阳 甘晓玲[1] 罗小东[1] Cai Liuyun;Hu Jianguo;Gong Yangyang;Gan Xiaolin;Luo Xiaodong(Department of Obstetrics and Gynecology,Second Affiliated Hospital of Chongqing Medical University)

机构地区：[1]重庆医科大学附属第二医院妇产科

出　　处：《重庆医科大学学报》2019年第8期1010-1015,共6页Journal of Chongqing Medical University

基　　金：国家自然科学基金资助项目（编号：81501220、81702557）;重庆市科委资助项目（编号：csct2015jcyjA10067）;重庆市卫计委资助项目（编号：2017MSXM031）

摘　　要：目的:探究Chemerin在多囊卵巢综合征(polycystic ovarian syndrome,PCOS)发病机制中的作用。方法:体外培养人卵巢颗粒细胞(COV434),分别下调及过表达COV434细胞中的Chemerin基因,采用ELISA测定培养液炎性因子白介素-6(interleukin-6,IL-6)、白介素-8(interleukin-8,IL-8)、白介素-10(interleukin-10,IL-10)及肿瘤坏死因子-α(tumor necrosis factor,TNF-α)浓度,EdU方法测定各组细胞增殖能力,流式细胞法测定细胞凋亡。结果:下调组(LV3-shChemerin-1、LV3-shChemerin-2)与阴性对照组(LV3-NC)相比,培养液中IL-6、IL-8、TNF-α浓度均明显降低,IL-10浓度明显升高,差异均有统计学意义(PIL-6=0.006,PIL-8=0.001,PTNF-α=0.012,PIL-10=0.002),颗粒细胞COV434增殖能力明显降低(P=0.004),凋亡明显增加(P=0.000)。过表达组(LV5-Chemerin)与阴性对照组(LV5-NC)相比,培养液中IL-6、IL-8、TNF-α明显升高,IL-10明显降低,差异均有统计学意义(PIL-6=0.044,PIL-8=0.008,PTNF-α=0.015,PIL-10=0.004),颗粒细胞COV434增殖能力明显提高(P=0.013),凋亡明显降低(P=0.029)。结论:Chemerin可能通过促进卵巢颗粒细胞炎症反应,并诱导颗粒细胞增殖抑制凋亡参与PCOS的发生发展。Objective:To investigate the role of Chemerin in the pathogenesis of polycystic ovarian syndrome(PCOS).Methods:Hu-man ovarian granulosa cells(COV434)were cultured in vitro.Down-regulation and overexpression of Chemerin were performed in the COV434 cells.Enzyme-linked immunosorbent assay was used to determine the concentrations of inflammatory factors such as interleukin-6(IL-6),interleukin-8(IL-8),and interleukin-10(IL-10),and tumor necrosis factor-α(TNF-α)in the culture medium.The EdU method and flow cytometry were used to measure cell proliferation ability and apoptosis,respectively.Results:Compared with the negative control group(LV3-NC),the down-regulation groups(LV3-shChemerin-1,LV3-shChemerin-2)had significantly lower concentrations of IL-6,IL-8,and TNF-αand a significantly higher concentration of IL-10 in the culture medium(PIL-6=0.006,PIL-8=0.001,PTNF-α=0.012,PIL-10=0.002),significantly lower proliferative capacity of COV434 cells(P=0.004),and signifi-cantly enhanced apoptosis(P=0.000).Compared with the negative control group(LV5-NC),the overexpression group(LV5-Chemerin)had significantly higher concentrations of IL-6,IL-8,and TNF-αand a significantly lower concentration of IL-10 in the culture medium(PIL-6=0.044,PIL-8=0.008,PTNF-α=0.015,PIL-10=0.004),significantly higher proliferative capacity of COV434 cells(P=0.013),and significantly reduced apoptosis(P=0.030).Conclusion:Chemerin plays a role in the development and progression of PCOS by promoting inflammatory response,inducing proliferation,and inhibiting apoptosis of ovarian granulosa cells.

关 键 词：人卵巢颗粒细胞COV434 CHEMERIN 白介素-6 白介素-8 白介素-10 肿瘤坏死因子-Α 

分 类 号：R711.75[医药卫生—妇产科学]