快速复温与慢速复温解冻对精子质量的影响  

Effect of fast thawing and slow thawing on sperm quality

作　　者：陈钢鑫 康跃凡[1] 林典梁[1] 孙艳[1] 邱华红[2] 曾凡湘[3] 郑备红[1] Chen Gangxin;Kang Yuefan;Lin Dianliang;Sun Yan;Qiu Huahong;Zeng Fanxiang;Zheng Beihong(Reproductive Center,Fujian Provincial Maternity and Children’s Hospital,Affiliated Hospital of Fujian Medical University;Clinical Laboratory,Fujian Provincial Maternity and Children’s Hospital,Affiliated Hospital of Fujian Medical University;Pharmacy Department,Fujian Provincial Maternity and Children’s Hospital,Affiliated Hospital of Fujian Medical University)

机构地区：[1]福建省妇幼保健院,福建医科大学附属医院生殖中心,福州350001 [2]福建省妇幼保健院,福建医科大学附属医院院检验科,福州350001 [3]福建省妇幼保健院,福建医科大学附属医院药剂科,福州350001

出　　处：《重庆医科大学学报》2019年第8期1064-1067,共4页Journal of Chongqing Medical University

基　　金：福建省妇幼保健院院内科研资助项目（编号：闽妇保院研17-30）

摘　　要：目的:探讨2种精液解冻方法对精子质量的影响。方法:筛选福建省妇幼保健院生殖中心的精液样本50例,每个样本均分2份后进行液氮蒸汽法冷冻,2组冷冻精液分别采用快速复温和慢速复温解冻后比较2组精液的解冻效果。解冻效果包括精子动力学参数,形态学参数,精子DNA碎片指数(sperm DNA fragmentation index,DFI),精子解冻后在室温下1、3、6、24 h后的活动率,非连续密度梯度离心后的精子动力学参数。结果:2组精子解冻后的精子动力学参数、精子正常形态率间的差异无统计学意义(P>0.01),快速组的精子DFI,精子头、体、尾部畸形率,畸形精子指数(teratozoospermia index,TZI),精子畸形指数(sperm deformity index,SDI)比慢速组更低(P<0.01),2组精子解冻后在室温保存1、3 h后精子动力学参数间的差异无统计学意义(P>0.01),而快速组解冻后在室温保存6、24 h后的精子动力学参数比慢速组更好(P<0.01)。2组进行非连续密度梯度离心后精子浓度、总活动精子数、前向运动精子活动率、精子回收率的差异无统计学意义(P>0.01)。结论:对于液氮蒸汽法冷冻的精液来说,快速复温解冻比慢速复温解冻对精子的损伤较小,其解冻效果更好。Objective:To investigate the effect of fast thawing and slow thawing methods on the sperm quality.Methods:A total of 50 semen samples were screened out from the Reproductive Center of Fujian Provincial Maternal and Children’s Hospital,and each sample was aliquoted into two parts and then cryopreserved by the liquid nitrogen vapor method.The two parts of the same semen were divided into two groups based on the methods used for thawing,i.e.,fast thawing or slow thawing,and were observed for the difference in the thawing effect.The thawing effect included sperm kinetic parameters,morphological parameters,sperm DNA fragmentation index(DFI),sperm motility at 1 h,3 h,6 h,and 24 h after thawing when kept at room temperature,and kinetic parameters of sperms after a discontinuous density gradient centrifugation.Results:There were no significant differences between the two groups in the sperm kinetic parameters and percentage of sperms with normal morphology after thawing(P>0.01).Compared with the slow thaw-ing group,the fasting thawing group had significantly reduced values of the following:sperm DFI,deformity rate of sperm head,body,and tail,teratozoospermia index(TZI),and sperm deformity index(SDI)(P<0.01).There were no significant differences between the two groups in the sperm kinetic parameters at 1 h and 3 h after thawing when kept at room temperature(P>0.01),but the fasting thawing group had significantly improved sperm kinetic parameters at 6 h and 24 h after thawing(P<0.01).There were no significant differences between the two groups in the sperm density,total motile sperm count,forward sperm motility,and sperm recovery after a discontinuous density gradient centrifugation(P>0.01).Conclusion:Fast thawing is better than slow thawing for semen cryopreserved by liquid nitrogen vapor due to less damage produced to sperms by the former.

关 键 词：精液冷冻 快速复温解冻 慢速复温解冻 精子质量 

分 类 号：R715.2[医药卫生—妇产科学]