桑白皮提取物中桑根酮C、桑根酮D及桑辛素的HPLC含量测定  

Simultaneous Determination of Sanganone C,Sanganone D and Morusin in Morus alba L.extract by HPLC

作　　者：魏敏 杜鸿灵 金玲[1] 施翠英[1] 张舜杰 李秋霞 王金兰 张旭[1] Wei Min;Du Hongling;Jin ling;Shi Cuiying;Zhang Shunjie;Li Qiuxia;Wang Jinlan;Zhang Xu(Pharmaceutical College,Chengdu University of Traditional Chinese Medicine,Ministry of Education Key Laboratory of Standardization of Chinese Herbal Medicine,State Key Laboratory Breeding Base of System atic Research,Development and Utilization of Chinese Medicine Resources,Chengdu 610075,China)

机构地区：[1]成都中医药大学药学院,教育部中药材标准化重点实验室,中药资源系统研究与开发利用省部共建国家重点实验室培育基地,成都中医大养生保健科技有限公司

出　　处：《亚太传统医药》2019年第10期46-49,共4页Asia-Pacific Traditional Medicine

基　　金：四川省科学技术厅扶贫项目(2017NFP0158)

摘　　要：目的:建立HPLC同时测定桑白皮提取物中桑根酮C、桑根酮D及桑辛素的含量测定方法,为其质量控制和评价提供参考。方法:采用InterSustain AQ-C18(250 mm×4.6 mm,5μm)色谱柱;流动相为乙腈(A)-0.1%醋酸水(B),梯度洗脱(0~20 min,45%~70%A;20~26 min,70%~80%A);流速1 mL·min^-1;柱温30℃;检测波长276 nm。结果:3种成分均能实现完全分离,且与其他成分分离度良好。3种成分分别在0.280~4.480μg、0.196~3.136μg、0.054~0.865μg范围内呈良好的线性关系,回归系数分别为1.0000、0.9992、0.9992。桑根酮C、桑根酮D及桑辛素的平均加样回收率(n=6)分别为95.41%、101.02%、101.27%,RSD分别为1.91%、2.31%、1.84%。结论:所用方法操作简便,测定准确,重复性好,可用于桑白皮提取物的质量控制与评价。Objective:To establish a HPLC method for the simultaneous determination of Sanganone C,Sanganone D,Morusin in the extract of Morus alba L.Methods:The quantitative analysis was carried out on a column of InterSustain AQ-C18(250 mm×4.6 mm,5 μm) by HPLC and the mobile phase consisted of acetonitrile(A)-0.1% acetic acid water(B).The gradient elution was 0~20 min,45%~70% A;20~26 min,70%~80% A under a flow rate of 1 mL·min^-1 at column temperature 30 ℃ with detection wavelength 276 nm.Results:All of the three components could be separated completely,and the separation degree from other components was good.A good linear relationship were found in the range of 0.280~4.480 μg,0.196~3.136 μg,0.054~0.865 μg.The regression coefficients were 1.000 0,0.999 2,0.999 2.The average recovery(n=6) of Sanganone C,Sanganone D and Morusin were 95.41%,101.02%,101.27%,respectively.The relative standard deviations were 1.91%,2.31%,1.84%,respectively.Conclusion:This method is simple,accurate and reproducible,and it can be used for the quality control of three components in the extract of Morus alba L..

关 键 词：桑白皮提取物 桑根酮C 桑根酮D 桑辛素 HPLC 

分 类 号：R284.2[医药卫生—中药学]