川芎提取物的分离及其对大鼠心肌缺血/再灌注损伤的药理作用  

Separation of Extracts from Chuanxiong Rhizoma and the Pharmacological Effects on Myocardium of Ischemia/Reperfusion Injury in Rats

作　　者：刘剑刚[1] 张大武[1] 刘雨杨 丰加涛[3] 李婕[4] 史大卓[1] 梁鑫淼[3] LIU Jiangang;ZHANG Dawu;LIU Yuyang;FENG Jiatao;LI Jie;SHI Dazhuo;LIANG Xinmiao(Department of Cardiology,Xiyuan Hospital,China Academy of Chinese Medical Sciences,Institute of Cardiovascular Diseases,China Academy of Chinese Medical Sciences,Beijing 100091,China;Vancouver School of Economics University of British Columbia,Vancouver V6T1Z4,Canada;Key Laboratory of Separation and Analytical Chemistry,Dalian Institute of Chemical Physics,Chinese Academy of Sciences,Dalian 116023,China;College of Biopharmaceutics,China Pharmaceutical University,Nanjing 210038,China)

机构地区：[1]中国中医科学院西苑医院心血管病中心/中国中医科学院心血管病研究所,北京100091 [2]Vancouver School of Economics University of British Columbia,Canada,Vancouver V6T1Z4 [3]中国科学院大连化学物理研究所分离分析化学重点实验室,辽宁大连116023 [4]中国药科大学生物制药学院,南京210038

出　　处：《中国现代应用药学》2019年第19期2369-2375,共7页Chinese Journal of Modern Applied Pharmacy

基　　金：国家重点基础研究发展计划项目(G19990544-05);中国科学院知识创新工程重要方向项目(KGCX2-SW-213)

摘　　要：目的评价采用大孔树脂方法分离的10种川芎组分对大鼠心肌缺血/再灌注损伤(myocardialischemia/reperfusion injury,I/R)后血栓形成、血小板功能和血管内皮因子的药理作用。方法川芎饮片采用水提取后醇沉方法,分别采用大孔树脂分离和高速逆流色谱法分离得到10种不同的川芎组分。清洁级Wistar大鼠,♀♂各半,采用(按0.009 mL·g-1体质量腹腔注射4%水合氯醛麻醉)结扎冠状动脉左前降支建立I/R模型。于结扎成功后(心电图显示)10 min时大鼠股静脉分别注射实验用药,川芎组分给药剂量为20mg·kg^-1;阳性对照组给予川芎嗪(盐酸四甲基吡嗪二水化合物),给药剂量为20 mg·kg^-1;假手术组和模型组给予等量生理盐水,每组10只。于缺血40 min再灌注120 min时取血,测定体外血栓形成的长度、湿重和干重,全血分离后放射免疫测定血浆内皮素-1含量,硝酸盐还原法测定血浆一氧化氮(NO)含量,比浊法测定血小板最大聚集率。结果与假手术组比较,模型组的血栓长度、湿重和干重均明显增加(P<0.05或P<0.01);与模型组比较,川芎组分②、③、⑤号组对血栓长度、湿重和干重均有不同程度的降低(P<0.05或P<0.01)。血管内皮因子和血小板聚集的结果表明,与假手术组比较,模型组内皮素-1水平明显上升(P<0.01),NO水平有降低趋势;与模型组比较,川芎组分⑤、⑥、⑧和⑩号组对大鼠血浆内皮素-1水平具有显著降低作用(P<0.05或P<0.01),对大鼠NO水平具有显著升高作用(P<0.05或P<0.01);川芎组分①至⑩号组均有显著降低大鼠血小板聚集率的作用(P<0.01)。结论通过HPLC和高速逆流色谱仪建立的川芎全组分指纹图谱具有良好的对应性,分离的川芎提取物的药理作用具有差异性且作用靶点不同,具有开发新产品的药效特征和研究的示范性。OBJECTIVE To evaluate the pharmacological effects of ten kinds of extracts from Chuanxiong Rhizoma,with macroporous resin method,on thrombosis,the platelet function and vascular endothelial factors in rat myocardium of ischemia/reperfusion injury(I/R).METHODS Ten different Chuanxiong Rhizoma component were extracted from Chinese herbal pieces Chuanxiong Rhizoma through water decoction alcohol sinking method and macroporous resin separation as well as high-speed countercurrent chromatography.Wistar rats of clean grade were randomly divided.I/R was established by ligation of coronary artery left anterior descending branch.Ten minutes after successful ligation,the different experimental drugs(20 mg·kg^-1 Chuanxiong Rhizoma component)were injected into rats from femoral vein;the rats in positive control group were injected with ligustrazine injection(including with 20 mg·kg^-1 tetramethylpyrazine hydrochloride dehydrate).The rats in sham group and the model group were injected with equivalent dosage of saline,ten rats in each group.The blood samples were extracted after 40 min myocardial ischemia and 120 min myocardial reperfusion,thrombus length,wet weight and dry weight of thrombosis in vitro were examined,plasma endothelin 1 was tested by radiation immunoassay method,plasma NO was tested by nitrate reduction method,the maximum platelet aggregation rate was tested by turbidimetric method.RESULTS Compared with sham control group,thrombus length,wet weight and dry weight of thrombosis in model group increased significantly(P<0.05 or P<0.01);Compared with model group,thrombus length,wet weight and dry weight of thrombosis in Chuanxiong Rhizoma component②group and③group as well as⑤group had different degrees of reduction(P<0.05 or P<0.01).Compared with sham control group,endothelin 1 level in model group increased significantly(P<0.01),NO level in model group decreased.Compared with model group,endothelin 1 level in Chuanxiong Rhizoma component⑤group,⑥group,⑧group and⑩group had significant reduction(P<0

关 键 词：川芎提取物 川芎嗪 心肌缺血 再灌注损伤 液相色谱 高速逆流色谱 中药指纹图谱 

分 类 号：R285.5[医药卫生—中药学]