DNA分子标记技术在甘草属植物鉴定中的应用研究进展  

Research Progress of DNA Molecular Marker Technology in Germplasm Identification of Glycyrrhiza Plants

作　　者：郝杰 刘刚 刘育辰 安兰兰 金文渊 袁思文 HAO Jie;LIU Gang;LIU Yuchen;AN Lanlan;JIN Wenyuan;YUAN Siwen(Guizhou University of Traditional Chinese Medicine,Guiyang 550025,China;National Engineering Research Center of Miao’s Medicines,Guiyang 550025,China;Guizhou Provincial Key Lab of Miao’s Medicines,Guiyang 550025,China)

机构地区：[1]贵州中医药大学,贵阳550025 [2]国家苗药工程技术研究中心,贵阳550025 [3]贵州省苗医药重点实验室,贵阳550025

出　　处：《中国现代应用药学》2019年第19期2485-2490,共6页Chinese Journal of Modern Applied Pharmacy

基　　金：国家自然科学基金项目(81473308)

摘　　要：近年来,利用DNA分子标记技术对甘草属植物进行遗传标记,以探讨该属植物分子层面差异性的相关研究取得了较为丰富的成果。但对于这方面的总结并不多见。本文以甘草属植物在遗传多样性、变异种质的鉴定和系统发育构建等方面研究的应用现状为线索,总结了甘草属植物研究中常用的DNA分子标记技术--随机扩增多态性DNA、扩增片段长度多态性、简单重复序列、间简单重复序列区间、目标起始密码子多态性和DNA条形码及其衍生技术的应用现状,以期为甘草属植物后期的研究提供一定的参考。In recent years, abundant achievements have been made in the study on the molecular diversity of Glycyrrhiza by DNA molecular marker technology. But the summary of this aspect is rare. With the application status in genetic diversity, identification of variant gerlogenetic construction of Glycyrrhiza for a clue, this paper summarizes the application of the commonly used DNA molecular markers: randomly amplified polymorphic DNA, amplified fragment length polymorphism, simple sequence repeat, inter simple sequence repeat, start codon target polymorphism, DNA barcoding and their derivative technologies in Glycyrrhiza, in order to provide a reference for the further study in Glycyrrhiza plants.

关 键 词：甘草属 DNA分子标记 遗传多样性 种质鉴定 系统发育 

分 类 号：R286.0[医药卫生—中药学]