3D打印聚己内酯/纳米羟基磷灰石复合支架与骨髓间充质干细胞的体外生物相容性  

In vitro biocompatibility of 3D printed polycaprolactone/nano-hydroxyapatite composite scaffold with bone marrow mesenchymal stem cells

作　　者：胡超然 邱冰 周祝兴 杨洋[3] 李佳 Hu Chaoran;Qiu Bing;Zhou Zhuxing;Yang Yang;Li Jia(Guizhou Orthopedics Hospital,Guiyang 550004,Guizhou Province,China;Peking University Health Science Center,Beijing 100871,China;Guizhou Medical University,Guiyang 550001,Guizhou Province,China)

机构地区：[1]贵州省骨科医院,贵州省贵阳市550004 [2]北京大学医学院,北京市100871 [3]贵州医科大学,贵州省贵阳市550001

出　　处：《中国组织工程研究》2020年第4期589-595,共7页Chinese Journal of Tissue Engineering Research

摘　　要：背景:聚己内酯/纳米羟基磷灰石复合材料是在常用骨组织工程材料基础上结合3D打印技术制备的新型复合支架材料,目前对于该复合材料的体外生物相容性研究较少。目的:通过体外实验探讨3D打印聚己内酯/纳米羟基磷灰石复合支架材料的细胞相容性。方法:利用3D打印技术分别制备聚己内酯及聚己内酯/纳米羟基磷灰石复合支架,表征两组材料的微观结构、孔隙率及力学性能。将大鼠骨髓间充质干细胞分别接种于两组支架表面,CCK-8法检测细胞增殖率,扫描电镜和Live/Dead染色观察细胞在支架上的生长情况。结果与结论:(1)两组支架均呈三维网状相互连通结构,纤维呈规律有序的排列、相互交错,纤维表面无空隙,纤维间距、直径较为均一;两组支架的孔隙率比较差异无显著性意义(P>0.05);复合支架的弹性模量高于单纯聚己内酯支架(P<0.05);(2)两组支架表面培养1 d的细胞增殖比较差异无显著性意义(P>0.05),复合支架表面培养4,7 d的细胞增殖快于单纯聚己内酯支架(P<0.05);(3)Live/Dead染色结果显示,两组材料均具有良好的细胞相容性,细胞活性较高,同时复合支架上的贴壁细胞更多一些;(4)扫描电镜显示,细胞在两种材料上生长形态良好,并紧密黏附于支架表面及微孔附近,同时可见分泌的细胞外基质呈丝状包绕于细胞周围;(5)结果表明,3D打印技术制备的聚己内酯/纳米羟基磷灰石复合支架孔隙较丰富,具备良好的力学性能,细胞相容性良好,可作为骨组织工程的支架材料。BACKGROUND: Polycaprolactone/nano-hydroxyapatite composite is a new composite scaffold material prepared based on common bone tissue engineering materials using 3D printing technology. At present, little is reported on the in vitro biocompatibility of the composite material. OBJECTIVE: To investigate the cytocompatibility of 3D printed polycaprolactone/nano-hydroxyapatite composite scaffolds. METHODS: Polycaprolactone and polycaprolactone/nano-hydroxyapatite composite scaffolds were prepared by 3D printing technology to characterize the microstructure, porosity and mechanical properties of the two materials. Rat bone m arrow mesenchymal stem cells were inoculated on the surface of the 3D-printed polycaprolactone and polycaprolactone/nano-hydroxyapatite composite scaffolds. Cell proliferation rate was detected by CCK-8 method. Cell growth on the scaffolds was observed by scanning electron microscopy and Live/Dead cell staining. RESULTS AND CONCLUSION: Two kinds of scaffolds had a three-dimensional network and interconnected structure. The fibers were arranged in a regular order and interlaced. There was no gap on the fiber surface, and the fiber spacing and diameter were relatively uniform. There was no significant difference in the porosity between two kinds of scaffolds(P>0.05). The elastic modulus of the composite scaffold was higher than that of the simple polycaprolactone scaffold(P<0.05). There was no significant difference in cell proliferation between two kinds of scaffolds after 1 day of culture. After 4 and 7 days of culture, cell proliferation on the composite scaffold was si gnificantly faster than that on the simple polycaprolactone scaffold(P<0.05). Live/Dead cell staining showed that both polycaprolactone and polycaprolactone/nano-hydroxyapatite composite scaffolds had good cytocompatibility and high cell viability. A larger number of cells adhered to the polycaprolactone/nano-hydroxyapatite composite scaffolds. Scanning electron microscopy showed that cells grew well on two kinds of scaffolds and d

关 键 词：骨组织工程 3D打印 聚已内酯 纳米羟基磷灰石 骨髓间充质干细胞 弹性模量 孔隙率 生物相容性 

分 类 号：R459.9[医药卫生—治疗学] R-331[医药卫生—临床医学]