阿托伐他汀对脓毒症大鼠心肌保护的研究  

Effects of Atorvastatin on levels of TNF-αand the study of Myocardial protection in septic rats

作　　者：王之余[1] 赫太平 顾金萍[1] 曲茂兴[1] 于健[1] Wang Zhiyu;He Taiping;Gu Jingping;Qv Maoxing;Yu Jian(Intensive Care Unit,the Second Affiliated Hospital of Dalian Medical University;Intensive Care Unit,Donggang Center Hospital,Dalian,China)

机构地区：[1]大连医科大学附属第二医院重症医学科 [2]东港市中心医院重症医学科

出　　处：《实用休克杂志（中英文）》2019年第4期213-218,共6页Journal of Practical Shock

摘　　要：目的探讨阿托伐他汀对脓毒症大鼠模型心肌保护作用和肿瘤坏死因子α(TNF-α)表达的影响。方法采用盲肠结扎法(CLP)制作脓毒症大鼠模型,随机分为对照组、脓毒症组、阿托伐他汀低剂量组和阿托伐他汀高剂量组,各组分别在术后0,3,6,12,24h各取五只大鼠采血,分离血浆检测TNF-α及cTnI的浓度,观察和比较各组大鼠活动度、小肠及心脏组织病理改变。采用SPSS 22.0软件包,于各时间点行重复测量资料方差分析。结果0h各组血浆TNF-α浓度比较差异无显著性意义(P>0.05)。对照组血浆TNF-α浓度变化不明显,在3、6、12、24h阿托伐他汀低剂量组、阿托伐他汀高剂量组较脓毒症组和对照组比较差异均有显著性意义(P<0.01),明显低于脓毒症组,阿托伐他汀高剂量组较阿托伐他汀低剂量组血浆TNF-α浓度明显降低(P<0.01),具有显著性差异。0h各组血浆cTnI浓度比较差异无显著性意义(P>0.05)。对照组血浆cTnI浓度变化不明显,在3、6、12、24h阿托伐他汀低剂量组、阿托伐他汀高剂量组较脓毒症组和对照组比较差异均有显著性意义(P<0.01),明显低于脓毒症组,在3h、6h、12h阿托伐他汀高剂量组较阿托伐他汀低剂量组血浆cTnI浓度明显降低(P<0.01),具有显著性差异。24h阿托伐他汀高剂量组较低剂量组血浆cTnI浓度无明显降低(P>0.05)。结论阿托伐他汀可以抑制TNF-α及cTnI的表达,减轻炎症反应,保护心肌。Objective To investigate the influence of atorvastatin on tumor necrosis factor-α(TNF-α)and myocardial protection of sepsis rats.Methods Sepsis models were established using male SD rats by cecal ligation and puncture(CLP).A total of 100 healthy rats were divided into 4 groups(n=25)randomly:sham-operation group,CLP group,low-dose atorvastatin group and high-dose atorvastatin group.Blood samples of 5 rats in each group were collected at postoperative 0、3、6、12 and 24 hours,to detect tumor necrosis factor-α(TNF-α)and cTnI.levels in plasma.Observe and compare the mobility of rats in each group、specimens of small intestine and cardiac were taken for histopathological examination by optical microscope.Results At 0 hour,plasmic TNF-αlevels in 4 groups were statistically equal(P>0.05).In plasma of sham-operation group,changes of TNF-αlevels were not obvious.Compared with CLP group,TNF-αlevels in low-dose and high-dose atorvastatin groups were both significantly lower(P<0.01)at postoperative 3,6,12 and 24 hours.And TNF-αlevels in high-dose atorvastatin group were significantly ower than those in low-dose group(P<0.01)at the 4 time points.At 0 hour,plasmic cTnI levels in 4 groups were statistica equal(P>0.05).In plasma of sham-operation group,changes of cTnI levels were not obvious.Compared with CLP group,cTnI levels in low-dose and high-dose atorvastatin groups were both significantly lower(P<0.01)at postoperative 3,6,12 and 24 hours.And cTnI levels in high-dose atorvastatin group were insignificantly ower than those in low-dose group(P>0.05)at the 4 time points.Conclusions Atorvastatin can inhibit the expression of TNF-αand cTnI in blood plasma of sepsis rats、reduce inflammatory reaction and protect the myocardium.

关 键 词：脓毒症 阿托伐他汀 肿瘤坏死因子Α 心肌肌钙蛋白I 心肌保护 

分 类 号：R54[医药卫生—心血管疾病]