CO2超临界流体色谱法同时测定莪术油中3个倍半萜类成分的含量  

Simultaneous Determination of Three Sesquiterpenoids in the Zedoary Turmeric Oil by CO2-Supercritical Fluid Chromatography

作　　者：唐登峰 马临科 龚青 方翠芬 TANG Dengfeng;MA Linke;GONG Qing;FANG Cuifen(Zhejiang Institute for Food and Drug Control,Hangzhou 310052,China)

机构地区：[1]浙江省食品药品检验研究院

出　　处：《中国现代应用药学》2019年第20期2539-2542,共4页Chinese Journal of Modern Applied Pharmacy

基　　金：浙江省科技计划项目(2016C37064)

摘　　要：目的建立CO2超临界流体色谱法测定莪术油中呋喃二烯、牻牛儿酮和莪术二酮含量的方法。方法采用ACQUITY UPC2 HSS C18 SB色谱柱(3.0 mm×150 mm,1.8μm),以CO2-乙腈为流动相,梯度洗脱;流速为1.0 mL·min^-1;检测波长为216 nm,柱温为55℃,背压为2 000 psi。结果呋喃二烯在2.67~1 337.26μg·mL^-1内线性关系良好(r=1.000),加样回收率为97.94%(n=6,RSD=1.50%)。牻牛儿酮在2.77~1 386.00μg·mL^-1内线性关系良好(r=1.000),加样回收率为96.07%(n=6,RSD=1.68%);莪术二酮在6.99~3 493.00μg·mL^-1内线性关系良好(r=1.000),加样回收率为99.33%(n=6,RSD=1.88%)。结论本方法快捷准确、稳定且绿色环保,可用于莪术油中上述3个倍半萜类成分的质量控制。OBJECTIVE A CO2-Supercritical Fluid Chromatography(CO2-SFC)method was established for the simultaneous determination of furanodiene,curdione and germacrone in the Zedoary Turmeric Oil.METHODS A UPC2 HSS C18 SB analytical column(3.0 mm×150 mm,1.8μm)was adopted and the mobile phase consisted of CO2-acetonitrile with linear gradient elution(0-8 min,99.5%→84%of CO2)at the flow rate of 1 mL·min^-1.The detection wavelength was 216 nm,the column temperature was maintained at 55℃,and the back pressure was was kept at 2 000 psi.RESULTS The linear range of furanodiene was 2.67-1 337.26μg·mL^-1(r=1.000),and the average recovery(n=6)was 97.94%with RSD of 1.50%.The linear range of curdione was 2.77-1 386.00μg·mL^-1(r=1.000),and the average recovery(n=6)was 96.07%with RSD of 1.68%.The linear range of germacrone was 6.99-3 493.00μg·mL-1(r=1.000),and the average recovery(n=6)was 99.33%with RSD of 1.88%.CONCLUSION The established method is rapid,accurate,and it can protect environment,which provides a new method for the quality control of furanodiene,curdione and germacrone in the Zedoary Turmeric Oil.

关 键 词：CO2超临界流体色谱法 呋喃二烯 牻牛儿酮 莪术二酮 莪术油 

分 类 号：R917.101[医药卫生—药物分析学]