铁蛋白笼形纳米颗粒应用于HIV-1 P24抗原高灵敏检测的研究  

Application of ferritin cage nanoparticles in high sensitivity detection of HIV-1 P24 antigen

作　　者：张利沙[1] 曾德军 ZHANG Lisha;ZENG Dejun(Department of Clinical Laboratory,Deyang Second People′s Hospital,Deyang,Sichuan 618000,China;Department of Microbiological Testing,Deyang Center for Disease Control and Prevention,Deyang,Sichuan 618000,China)

机构地区：[1]德阳市第二人民医院检验科,四川德阳618000 [2]德阳市疾病预防控制中心微生物检验所,四川德阳618000

出　　处：《国际检验医学杂志》2019年第21期2572-2575,共4页International Journal of Laboratory Medicine

基　　金：德阳重点科学技术研究项目(2018SZS061)

摘　　要：目的构建以铁蛋白笼形纳米颗粒为基础的分子展示平台,并应用于HIV-1 P24抗原检测,实现低丰度、高灵敏检测。方法采用分子生物学手段,构建生物素耦联的铁蛋白笼形纳米颗粒,基于链霉亲和素与生物素特异性结合,将其应用于HIV-1 P24抗原的酶联免疫吸附测定(ELISA)检测中,并测试其检测灵敏度。结果构建出具有链霉亲和素特异性结合能力的生物素化铁蛋白笼形纳米颗粒,应用到HIV-1的P24抗原ELISA检测中,实现0.1 ng/mL的检测下限,显著提高检测灵敏度。结论成功建立基于铁蛋白笼形纳米结构的分子展示平台,以HIV-1 P24抗原ELISA检测为例,该平台能够应用于低丰度、高灵敏检测。Objective To construct a molecular display platform based on ferritin cage nanoparticles and apply it to HIV-1 P24 antigen detection to achieve low abundance and high sensitivity.Methods Biotin-coupled ferritin cage nanoparticles were constructed by molecular biology.Based on the specific binding of streptavidin and biotin,they were applied to the detection of HIV-1 P24 antigen by enzyme-linked immunosorbent assay(ELISA)and the detection sensitivity was tested.Results The cage-shaped biotinylated ferritin nanoparticles with the specific binding ability of streptavidin were constructed and applied to the detection of HIV-1 P24 antigen by ELISA.The detection limit was 0.1 ng/mL,and the detection sensitivity was significantly improved.Conclusion A molecular display platform based on ferritin cage nanostructure was successfully established.Taking ELISA detection of HIV-1 P24 antigen as an example,the platform can be applied to low abundance and high sensitivity detection.

关 键 词：铁蛋白 纳米颗粒 HIV-1 P24抗原 高灵敏检测 

分 类 号：R446.61[医药卫生—诊断学]