高效液相色谱法测定健儿消食口服液中黄芩苷含量  

Content Determination of Baicalin in JianEr XiaoShi Oral Solution by HPLC

作　　者：徐静娟 栗金权 XU Jing-juan;LI Jin-quan(Changzhou Children′s Hospital,Changhzou 213003,China)

机构地区：[1]常州市儿童医院

出　　处：《海峡药学》2019年第10期89-90,共2页Strait Pharmaceutical Journal

摘　　要：目的建立高效液相色谱法测定健儿消食口服液中黄芩苷的含量。方法采用C 18色谱柱(4.6×250mm,5μm),以甲醇-水-磷酸(47∶53∶0.2)为流动相,流速为1.0mL·min^-1,检验波长为277nm;柱温:30℃;进样量:10μL。结果黄芩苷在10.1~80.8μg·mL^-1的浓度范围内有良好的线性关系(r=0.9996)。通过加样回收率试验对方法准确度进行验证,结果平均回收率为100.76%,RSD为0.48%(n=6)。结论该方法简便实用,结果准确,可用于健儿消食口服液中黄芩苷的含量测定。OBJECTIVE To establish a HPLC method for the content determination of JianEr XiaoShi oral solution.METHODS A column of WATERS symmetry C 18(4.6×250mm,5μm)was used with a mobile phase of methanol-water-phosphoric acid(47∶53∶0.2),the sample was determined at wavelength of 277nm and the temperature of column was 30℃,the flow rate was 1.0mL·min^-1 and the sample size was 10μL precisely.RESULTS Baicalin had a good linear relationship in the concentration range of 10.1-80.8μg·mL^-1(r=0.9996);the average recovery(n=6)was 100.76(RSD%=0.48%).CONCLUSION The method is simple,rapid,accurate,and suitable for the content determination of Baicalin in JianEr XiaoShi oral solution.

关 键 词：高效液相色谱法 健儿消食口服液 黄芩苷 

分 类 号：R927[医药卫生—药学]