开放实验项目“中药制剂特征成分HPLC分析方法的建立”的设计  

Design of Open Experiment Project ‘Establishment of HPLC Analysis Method for Characteristic Components of Traditional Chinese Medicine’

作　　者：廖晓燕[1] 梁淑彩[1] 朱瑜超 肖玉秀[1] LIAO Xiaoyan;LIANG Shucai;ZHU Yuchao;XIAO Yuxiu(School of Pharmaceutical Science,Wuhan University,Wuhan 430071,China)

机构地区：[1]武汉大学药学院

出　　处：《实验科学与技术》2019年第5期94-98,共5页Experiment Science and Technology

基　　金：武汉大学教学改革研究项目(2019JG017,JG201670,JG201469);武汉大学实验技术项目(WHU-2014-SYJS-07);武汉大学医学部教学改革研究项目(2016017)

摘　　要：该文以鼻渊舒口服液为例,建立同时测定鼻渊舒口服液中栀子苷和黄芩苷含量的双波长HPLC法,并进行专属性、线性与范围、精密度、准确度、耐用性的方法学验证,以及实际样品的测定。其结果表明栀子苷和黄芩苷分别在8. 03~200. 85μg·mL^-1(r=1. 0000)和2. 86~71. 47μg·mL^-1(r=0. 9997)浓度范围内线性关系良好。该方法精密度良好,重复性RSD均小于2. 05%;加样回收率在91. 69%~107. 66%之间。该实验项目难度适中,可以着眼于不同的中药制剂,既可以保持一定的创新性,又能保证实验的成功率,提高学生实验热情,同时能够培养学生的科研素养,适合作为药物分析实验的开放性实验教学项目。Taking Biyuanshu oral liquid as an example,a dual-wavelength HPLC method for simultaneous determination of characteristic components of traditional Chinese medicine geniposide and baicalin in Biyuanshu oral liquid was established. Methodologies for specificity,linearity and range,precision,accuracy,and durability were validated and actual samples were determined. The results showed that the relationship between geniposide and baicalin was linear in the range of density 8. 03~200. 85μg·mL^-1(r =1. 0000)and 2. 86~71. 47μg·mL^-1(r = 0. 9997). The precision of the method is good,the reproducible RSD is less than 2. 05%;the recovery rate of the sample is between 91. 69% and 107. 66%. The experimental project is moderately difficult and can focus on different traditional Chinese medicine preparations,which can not only maintain a certain degree of innovation,but also ensure the success rate of the experiment. The project also can improve the enthusiasm of the students,and at the same time cultivate the scientific research literacy of the students,which is suitable used for open experiment teaching project of drug analysis experiments.

关 键 词：鼻渊舒口服液 高效液相色谱法 栀子苷 黄芩苷 

分 类 号：R284.1[医药卫生—中药学]