PM2.5暴露对SD大鼠甲状腺功能的影响  

Effect of PM2.5 exposure on thyroid function in SD rats

作　　者：郎佳楠 张宝玉[1] 赵冬[1] 关龙飞 付颖[1] Lang Jianan;Zhang Baoyu;Zhao Dong;Guan Longfei;Fu Ying(Beijing Key Laboratory of Diabetes Research and Care,Center for Endocrine Metabolism and Immune Diseases,Luhe Hospital,Capital Medical University,Beijing 101149,China;China-America Institute of Neuroscience,Beijing Luhe Hospital,Capital Medical University,Beijing 101149,China)

机构地区：[1]首都医科大学附属北京潞河医院内分泌代谢与免疫中心,101149 [2]首都医科大学附属北京潞河医院中美神经研究所,101149

出　　处：《中华内分泌代谢杂志》2019年第10期874-877,共4页Chinese Journal of Endocrinology and Metabolism

摘　　要：目的探讨PM2.5暴露对雄性SD大鼠甲状腺功能的影响。方法成年雄性SD大鼠分为洁净空气(FA)组和PM2.5组,暴露12周后处死大鼠,取大鼠血浆和甲状腺组织。HE染色观察甲状腺组织的形态和病理变化。酶联免疫吸附法(ELISA)定量分析血浆中三碘甲状腺原氨酸(T3)、游离T3(FT3)、甲状腺素(T4)、游离T4(FT4)、抗甲状腺球蛋白抗体(TGAb)、抗甲状腺过氧化物酶抗体(TPOAb)、促甲状腺素(TSH)、肿瘤坏死因子α(TNF-α)和白细胞介素6(IL-6)水平。结果经12周处理后,2组大鼠的体重无统计学差异。2组大鼠甲状腺组织HE染色后无明显病理改变和统计学差异。2组大鼠血浆T4、T3、FT3、TNF-α和IL-6水平差异均无统计学意义,但与FA组大鼠相比,PM2.5暴露组大鼠血浆FT4水平显著降低[(3.81±1.23)对(6.02±2.40)pmol/L,P<0.01],TSH水平升高[(5.63±1.74)对(4.29±1.37)mU/L,P<0.05]。结论为期12周的PM 2.5暴露可引起大鼠FT4水平下降,TSH水平升高。Objective To investigate the effect of PM2.5 exposure on the thyroid function in male SD rats.Methods Adult Sprague-Dawley rats were divided into"filtered air"(FA)and"PM2.5"groups.These rats exposed to the 2 kinds of air for 12 weeks were killed and their plasma and thyroid tissues were taken for investigation.The morphological status and pathological changes of thyroid tissue were observed after H&E staining.The levels of triiodothyronine(T3),free triiodothyronine(FT3),thyroxine(T4),free thyroxine(FT4),anti-thyroglobulin antibody(TGAb),anti-thyroid peroxidase antibody(TPOAb),thyrotropin(TSH),tumor necrosis factorα(TNF-α),and interleukin-6(IL-6)in rats were measured by ELISA.Results The results showed that there was no significant difference in body weight between two groups after 12 weeks of exposure to either filtered air or PM2.5.No pathological changes and significant differences were observed in the thyroid tissues of two groups after HE staining.There were no significant differences in plasma levels of T4,T3,FT3,TNF-α,and IL-6 between two groups.However,compared with rats exposed to filtered air,rats exposed to PM2.5 had significantly lower plasma FT4[(3.81±1.23)vs(6.02±2.40)pmol/L,P<0.01]and higher TSH[(5.63±1.74)vs(4.29±1.37)mU/L,P<0.05]levels.Conclusion The exposure to PM2.5 for a period of 12 weeks may lead to the decrease of FT4 and increase of TSH in rats.

关 键 词：PM2.5 甲状腺 促甲状腺素 

分 类 号：R58[医药卫生—内分泌]