聚乙二醇-硫量子点检测胰岛素  

Detection of insulin by PEG-Sulfur quantum dot

作　　者：梁慧凤 巩岩[1] 许强[1] 栗东霞[1] 闫桂琴[1] LIANG Hui-feng;GONG Yan;XU Qiang;LI Dong-xia;YAN Gui-qin(School of Life Science,Shanxi Normal University,Linfen 041004)

机构地区：[1]山西师范大学生命科学学院

出　　处：《分析试验室》2019年第10期1168-1172,共5页Chinese Journal of Analysis Laboratory

基　　金：国家自然科学基金(31700876,31700862);山西省自然科学基金(201601D021106);山西师范大学研究生科技创新项目(01053013)资助

摘　　要：以聚乙二醇-400为钝化剂,合成了一种新型纳米材料—聚乙二醇-硫量子点,具有良好的水溶性和优异的光稳定性,通过透射电子显微镜对其形态学进行表征,通过紫外吸收光谱和荧光光谱表征其光谱学特性。通过优化,确定聚乙二醇-硫量子点的激发波长为400 nm,发射峰位于490 nm,基于荧光增强法检测胰岛素,胰岛素浓度在43~346μmol/L范围内与荧光增强效率具有很好的线性关系(R^2=0.9949),检测限为11.71μmol/L。该荧光传感平台可以应用于实际样品中检测胰岛素。A novel nanomaterial-sulfur quantum dot(SD)with good water solubility and excellent light stability was synthesized with polyethylene glycol-400(PEG)as a passivating agent.Its morphology was characterized by transmission electron microscopy,and its spectral characteristics were characterized by ultraviolet absorption spectroscopy and fluorescence spectroscopy.After optimization,the excitation wavelength of PEG-SDs was determined to be 400 nm,and the emission peak was located at 490 nm.Detection of insulin was achieved based on the fluorescence enhancement method with a good linear relationship of fluorescence enhancement efficiency and insulin concentration within the range of 43~346μmol/L(R2=0.9949),and the detection limit was 11.71μmol/L.The fluorescence sensor platform can be used to detect insulin in real samples.

关 键 词：聚乙二醇-硫量子点 胰岛素 荧光增强 

分 类 号：R446[医药卫生—诊断学] O657.3[医药卫生—临床医学]