超高效液相色谱荧光检测法检测禽肌肉中甲砜霉素  

Determination of thiamphenicol residues in poultry muscle by ultra performance liquid chromatography with fluorescence detectior

作　　者：王波 赵霞[2,3] 谢恺舟 郭亚文[2,3] 卜晓娜 刘楚君[2,3] 刁志祥 王旭堂[2,3] 张培杨 张跟喜 张涛 吴海清 刘学忠 WANG Bo;ZHAO Xia;XIE Kai-zhou;GUO Ya-wen;BU Xiao-na;LIU Chu-jun;DIAO Zhi-xiang;WANG Xu-tang;ZHANG Pei-yang;ZHANG Gen-xi;ZHANG Tao;WUHai-qing;LIU Xue-zhong(College of Veterinary Medicine,Yangzhou University,Yangzhou 225009;Joint International Research Laboratory of Agriculture&Agri-Product Safety,Yangzhou University,Yangzhou 225009;College of Animal Science and Technology,Yangzhou University,Yangzhou 225009)

机构地区：[1]扬州大学兽医学院,扬州225009 [2]教育部农业与农产品安全国际合作联合实验室,扬州225009 [3]扬州大学动物科学与技术学院,扬州225009

出　　处：《分析试验室》2019年第10期1189-1193,共5页Chinese Journal of Analysis Laboratory

基　　金：国家现代农业产业技术体系项目(CARS-41-G23);江苏高校优势学科建设工程资助项目(201901);扬州大学“高端人才支持计划”项目(201801);江苏省农业科技自主创新基金项目(CX(18)3022);扬州大学研究生国际学术交流专项基金项目(201905)资助

摘　　要：建立了鸡肌肉、鸭肌肉和鹅肌肉中甲砜霉素(TAP)残留的超高效液相色谱荧光检测方法。禽肌肉样品采用了加速溶剂萃取(ASE)进行了提取、净化,乙酸乙酯饱和的正己烷去脂,净化后用超高效液相色谱荧光检测器(UPLC-FLD)进行分析。空白禽肌肉样品中TAP的添加量在LOQ^250.0μg/kg范围内,线性关系良好,决定系数在0.9997~0.9998之间。TAP添加水平为LOQ,0.5,1.0和2.0倍的最离残留限量(MRL)时,平均回收率在81.6%~97.2%范围内,相对标准偏差(RSD)在2.3%~4.0%之间,日内相对标准偏差在2.4%~4.9%之间,日间相对标准偏差在3.1%~5.3%之间。TAP在禽肌肉中的检测限(LOD)和定量限(LOQ)的范围分别为3.0~3.3μg/kg和8.5~9.0μg/kg。方法可用于检测禽肌肉中甲砜霉素的残留。The method for the determination of thiamphenicol(TAP)residues in chicken,duck and goose muscle was established by using ultra-performance liquid chromatography with fluorescence detector(UPLC-FLD).Poultry muscle samples were extracted and purified by accelerated solvent extraction(ASE),and the fat was removed by saturated ethyl acetate and n-hexane.The purified products were detected by UPLC-FLD system.A satisfied linearity was obtained when the added concentrations of TAP in the blank sample of poultry muscle were in the range from LOQ to 250μg/kg,the determination correlations were in the range of 0.9997-0.9998.when the added concentrations of TAP were the LOQs amount,0.5,1.0 and 2.0 MRL,the average recoveries were 81.6%-97.2%,the relative standard deviations(RSDs)were 2.3%-4.0%,and the corresponding intra-day RSDs and inter-day RSDs were 2.4%-4.9%and 3.1%-5.3%,respectively.The method was successfully applied to detect ATP resides in poultry muscle.

关 键 词：禽肌肉 甲砜霉素 残留 超高效液相色谱荧光检测法 

分 类 号：S859.84[农业科学—临床兽医学] TS207.5[农业科学—兽医学]