慢性乙肝病毒感染患者血清免疫调节因子检测及基因多态性分析  

Investigation of the value of serum immunoregulatory factors in patients with a chronic hepatitis B virus infection

作　　者：连桂秀 毛华[1] 卢敏[1] 靳丹丹[1] LIAN Gui-xiu;MAO Hua;LU Min;JIN Dan-dan(Zhujiang Hospital,Southern Medical University,Guangzhou,China 510280)

机构地区：[1]南方医科大学珠江医院

出　　处：《中国病原生物学杂志》2019年第9期1104-1107,1111,共5页Journal of Pathogen Biology

摘　　要：目的检测不同人群血清免疫调节因子水平,为临床检测和治疗慢性乙肝病毒感染提供依据。方法 2013年6月-2016年6月广东省南方医科大学珠江医院诊治的慢性HBV感染患者116例。按HBV感染后临床表现分为:慢性HBV携带组22例,慢性乙型肝炎组39例和乙型肝炎肝硬化组55例。对照组选取健康体检者29例。采集受检者静脉血,分离血清,采用ELISA检测HBsAg、HBeAg、抗HBs和抗HCV等,采用双抗体夹心ABC-ELISA法检测IFN-γ、IL-4及IL-12等细胞因子,采用全自动生化仪检测肝功能指标。采用聚乙二醇沉淀法浓缩血清中的HBV。碱变形法提取血DNA,采用PCR热扩增HBV DNA。提取人全血DNA,采用PCR热扩增TNF-α-857和TNF-α-863并进行酶切和基因多态性分析。结果 HBV携带组、慢性乙型肝炎组、肝硬化组和对照组的ALB(g/L)分别为:38.78±9.56、23.8±8.2、23.6±6.4和47.43±9.56,组间比较差异有统计学意义(P<0.05);ALT(U/L)浓度分别为:98.5±32.8、187.7±37.1、102.6±41.9和32.1±11.8,组间比较差异有统计学意义(P<0.05);TBIL(μmol/L)分别为:68.72±32.51、72.2±47.86、73.6±42.9和15.39±5.33,组间比较差异有统计学意义(P<0.05);IL-4(pg/ml)分别为:108.71±31.52、96.89±26.29、92.74±28.47和28.43±5.56,组间比较差异有统计学意义(P<0.05);IL-12(pg/ml)分别为:33.96±7.38、58.31±10.12、43.29±9.53和17.45±7.82,组间比较差异有统计学意义(P<0.05)。IFN-γ(pg/ml)分别为:48.65±11.57、73.51±20.82、56.31±42.9和22.71±5.39,组间比较差异有统计学意义(P<0.05)。TNF-α-857基因多态性检测HBV携带组CC纯合型15例,TT纯合型1例,CT杂合型6例;慢性乙型肝炎组CC纯合型26例,TT纯合型3例,CT杂合型10例;肝硬化组CC纯合型36例,TT纯合型4例,CT杂合型15例;对照组CC纯合型15例,TT纯合型3例,CT杂合型11例。TNF-α-863基因多态性检测,HBV携带组CC纯合型14例,AA纯合型2例,CA杂合型6例;慢性乙型肝炎组CC纯合型24例,AA纯合型3例,CA杂合�Objectives To investigate levels of serum immunoregulatory factors in different groups in order to provide evidence for clinical detection and treatment of chronic hepatitis B virus(HBV) infection. Methods Subjects were 116 patients with a chronic HBV infection seen at Zhujiang Hospital of Southern Medical University in Guangdong Province from June 2013 to June 2016. Based on clinical manifestations of an HBV infection, 22 patients were chronic HBV carriers, 39 had chronic hepatitis B, and 55 had hepatitis B-related cirrhosis. Twenty-nine individuals undergoing a checkup served as the control group. HBsAg, HBeAg, anti-HBs, and anti-HCV were detected using ELISA. The cytokines IFN-gamma, IL-4, and IL-12 were detected using double antibody sandwich ABC-ELISA. Liver function was determined by an automated biochemical analyzer. FibroTouch was used to measure the hardness of the right lobe of the liver. HBV in serum samples was concentrated using polyethylene glycol precipitation, and DNA was extracted using alkali deformation. Serum HBV DNA was quantitatively measured using a polymerase chain reaction(PCR). Human whole blood DNA was extracted, and TNF-alpha-857 and TNF-alpha-863 were amplified using PCR and analyzed with restriction enzyme digestion and gene polymorphism. Results The level of ALB(g/L) was 38.78±9.56 in HBV carriers, 23.8±8.2 in patients with chronic hepatitis B, 23.6±6.4 in patients with cirrhosis, and 47.43±9.56 in the control group. Levels of ALB(g/L) differed significantly between the groups(P<0.05). The level of ALT(U/L) was 98.5±32.8 in HBV carriers, 187.7±37.1 in patients with chronic hepatitis B, 102.6±41.9 in patients with cirrhosis, and 32.1±11.8 in the control group. Levels of ALB(U/L) differed significantly between the groups(P<0.05). The level of TBIL(μmol/L) was 68.72±32.51 in HBV carriers, 72.2±47.86 in patients with chronic hepatitis B, 73.6±42.9 in patients with cirrhosis, and 15.39±5.33 in the control group. Levels of TBIL(μmol/L) differed significantly between the grou

关 键 词：乙型肝炎 免疫调节因子 检测 

分 类 号：R373.21[医药卫生—病原生物学]