小鼠腹股沟皮下脂肪组织与肾被膜部位移植胰岛降血糖功能的比较  

Comparison of blood glucose-lowering function of transplant islets between subcutaneous adipose tissues of inguinal region and renal capsule in mice

作　　者：彭园征 邹志成[2] 陈姣[2] 陆赢[2] 张汉成[2] 蔡志明[2] 牟丽莎[1,2] Peng Yuanzheng;Zou Zhicheng;Chen Jiao;Lu Ying;Zhang Hancheng;Cai Zhiming;Mou Lisha(ZhongShan School of Medicine,Sun Yat-sen University,Guangzhou 510080,China)

机构地区：[1]中山大学中山医学院,广州510275 [2]深圳市第二人民医院

出　　处：《器官移植》2019年第6期684-689,共6页Organ Transplantation

基　　金：国家重点研发计划(2017YFC1103704);深圳市“医疗卫生三名工程”(SZSM201412020);广东省高水平医院建设专项经费(2019);深圳市医学高水平学科建设(2016031638);深圳市科技计划(JCJY20160229204849975、GJHZ20170314171357556);深圳市卫生计生系统科研项目(SZXJ2017021、SZXJ2018059)

摘　　要：目的比较腹股沟皮下白色脂肪组织与肾被膜下移植胰岛治疗小鼠1型糖尿病的效果。方法将1型糖尿病模型小鼠分为白色脂肪组(10只)和肾被膜组(10只)接受胰岛移植。胰岛分离和纯化后,分别移植到腹股沟皮下白色脂肪组织和肾被膜下位点。术后持续监测两组受体小鼠的随机血糖水平、糖耐量功能,移植后100d摘取两组存活受体小鼠的胰岛移植物进行组织病理学检查。结果白色脂肪组有6只受体小鼠的血糖在移植后1个月恢复正常水平,其余4只受体小鼠一直维持着高血糖状态,陆续在监测结束前发生死亡;肾被膜组10只受体小鼠的血糖均在移植后10d内恢复正常。白色脂肪组和肾被膜组受体小鼠的胰岛移植物均能降低血糖水平,但白色脂肪组胰岛移植物需要较长的时间方能发挥降血糖功能。肾被膜组小鼠的糖耐量功能优于白色脂肪组小鼠(P<0.05)。组织病理学检查显示白色脂肪组和肾被膜组胰岛移植物的胰岛素表达均正常。结论腹股沟皮下白色脂肪组织部位移植胰岛能有效地发挥调控血糖变化的功能,尽管其降血糖功能稍弱于肾被膜下部位,但具有贴近理想胰岛移植位点的众多优势,是一个值得研究的胰岛移植替代部位。Objective To compare the effect of transplant islets between the subcutaneous inguinal white adipose tissues and renal capsule in the treatment of type 1 diabetes mellitus in mouse models. Methods The mice with type 1 diabetes mellitus undergoing islet transplantation were divided into the white adipose group (n=10) and renal capsule group (n=10). The islets were isolated, purified and transplanted to the subcutaneous white adipose tissues of inguinal region and renal capsule. The random blood glucose level and glucose tolerance function of the recipient mice in two groups were continuously monitored after operation. Islet grafts of the surviving recipient mice were harvested at postoperative 100 d for histopathological examination. Results In the white adipose group, the blood glucose levels of 6 recipient mice were restored to normal at 1 month after transplantation, whereas the blood glucose levels of the other 4 recipient mice were high, which died before the end of monitoring. In the renal capsule group, the blood glucose levels of 10 recipient mice returned to normal within 10 d after transplantation. Islet grafts of the recipient mice in two groups could lower the blood glucose levels, whereas the islet grafts in the white adipose group required a longer time to exert the effect. The glucose tolerance function of the mice in the renal capsule group was significantly better than that of those in white adipose group (P<0.05). Histopathological examination demonstrated that the insulin of the islet grafts was normally expressed in two groups. Conclusions The islets transplanted into the subcutaneous white adipose tissues of inguinal region can play an effective role in regulating the changes of blood glucose level. Although the blood glucose-lowering function is slightly weaker than that of the islets graft in the renal capsule, it has multiple advantages resembling the ideal islet transplantation sites, which is a promising replacement site for islet transplantation.

关 键 词：1型糖尿病 胰岛移植 腹股沟皮下白色脂肪组织 肾被膜 糖耐量功能 血糖 胰腺 

分 类 号：R617[医药卫生—外科学] R587.1[医药卫生—临床医学]