拮抗菌株JA38对人参锈腐病室内毒力及田间防治效果  

Indoor Toxicity and Field Control Effect of Antagonistic Strain JA38 on Ginseng Rust Rot

作　　者：姜云[1] 张子恒 陈长卿[2] 陈琳 闫东 王润林 唐浩 孙佳琪 美合日阿依 JIANG Yun;ZHANG Zi-heng;CHEN Chang-qing;CHEN Lin;YAN Dong;WANG Run-lin;TANG Hao;SUN Jia-qi;MEI He-ri-a-yi(Jilin Agricultural University,College of Life Science,Changchun 130118,China;Jilin Agricultural University,College of Agronomy,Changchun 130118,China)

机构地区：[1]吉林农业大学生命科学学院,长春130118 [2]吉林农业大学农学院,长春130118

出　　处：《农药》2019年第10期769-772,共4页Agrochemicals

基　　金：国家重点研发计划资助(2017YFD0201106,2018YFD0201100);农业部东北作物有害生物综合治理重点实验室开放课题(DB2018);吉林省科技厅市县科技进步推进项目(20191001016XH)

摘　　要：[目的]明确菌株JA38对人参锈腐病的室内毒力及田间防治效果。[方法]采用菌丝生长速率法测定菌株JA38对人参锈腐病菌的室内抑制效果,灌根法进行了人参锈腐病的田间药效试验。[结果]室内毒力测定结果表明:当菌株JA38发酵液浓度为10^9cfu/mL,其抑菌率达100%,EC50值为1.4×10^6cfu/mL;且对人参锈腐病菌的产孢量、孢子萌发、菌丝生长均有抑制作用;显微观察发现菌株JA38能够导致人参锈腐病菌菌丝断裂、分支增多和生长畸形。田间试验结果表明:含有0.25×10^7-1.0×10^7cfu/mL的JA38菌株发酵液防治效果达40.96%-60.77%。[结论]生防菌株JA38具有较好防治人参锈腐病的应用前景。[Aims] This study aims to determine the indoor toxicity and field control effect of Antagonistic strain JA38 on Ginseng rust rot. [Methods] Indoor virulence of Antagonistic strain JA38 on Ginseng rust rot was investigated by mycelial growth rate method. Field control effect on Ginseng rust rot was determined by rooting method. [Results] The toxicity measurement indicated that when the concentration of the fermentation broth of the strain JA38 was 10^9 cfu/mL,the inhibition rate was 100%, EC50 is 1.4×10^6 cfu/mL. The sporulation, spore germination and mycelial growth of ginseng rust rot were inhibited. Microscopic observation found that strain JA38 can cause hyphae rupture, branching increase and growth malformation of ginseng rust rot. The results of field experiment showed that the control effect of fermentation broth containing JA38 strain containing 0.25 ×10^7-1.0 ×10^7 cfu/mL was 40.96-60.77%. [Conclusions] The biocontrol strain JA38 has a good application prospect for the prevention and control of ginseng rust rot.

关 键 词：拮抗菌株 室内毒力 抑制效果 田间防效 

分 类 号：S482.2[农业科学—农药学]