以厚朴温中汤为例探讨煮散类经典名方物质基准制备工艺研究  

Study on benchmark preparation technology of boiled and powdered classical prescriptions by taking Houpo Wenzhong Decoction as an example

作　　者：罗菊元 陈功森[1] 刘冬涵 莫雨佳 喻祥龙 杜守颖[1] 白洁[1] 陆洋[1] 王宇明 刘存 LUO Ju-yuan;CHEN Gong-sen;LIU Dong-han;MO Yu-jia;YU Xiang-long;DU Shou-ying;BAI Jie;LU Yang;WANG Yu-ming;LIU Cun(School of Chinese Maieria Medial,Beijing University of Chinrse Medicine,Beijing 100029,China;Yifan Pharmaceutical Co.,Ltd.f Lin'an 311300,China)

机构地区：[1]北京中医药大学中药学院,北京100029 [2]亿帆医药股份有限公司,浙江临安311300

出　　处：《中国中药杂志》2019年第18期3994-3999,共6页China Journal of Chinese Materia Medica

基　　金：中央高校横向课题项目(2020071720252);中央高校基本科研业务费专项(2018-JYB-XJQ004)

摘　　要：近年来,经典名方的开发与应用备受关注,然而古今差异导致实际工作的开展困难重重。该文以小见大,以厚朴温中汤为例,在文献调研充足的基础上,考察了煮散的粒度、加水量、煎煮时间等关键工艺及样品检测前处理方法。实验结果表明,不同粒度(2,3~5 mm)的饮片粉末和不同煎煮时间(30,60 min)煎煮后,指标成分浓度整体无显著性差异,但饮片粉末粒度为2 mm及煎煮30 min时所得出膏率均相对较高;煎煮时随着加水量的增加,指标成分浓度和出膏率均呈不同程度的上升趋势;厚朴温中汤物质基准样品检测前采用一定比例的甲醇水溶液作为复溶溶剂,可兼顾方中水溶性成分和脂溶性成分的谱图信息,有助于更全面的展示处方化学成分信息的全貌。同时对《古代经典名方目录(第一批)》中煮散经方进行多层次整理归纳,多角度分析煎煮工艺的关键影响因素,提出初步研究建议。以期为厚朴温中汤质量标准的建立和煮散类经典名方的开发研究与临床应用提供一定方向和参考。In recent years,the development and application of classical famous prescriptions have attracted much attention.However,the differences between ancient and modern conditions lead to difficulties in carrying out practical work.In this paper,with Houpu Wenzhong Decoction as an example,the key technologies of boiling granularity,water addition,boiling time and sample pretreatment methods were investigated on the basis of sufficient literature research.The experimental results showed that there was no significant difference in the concentration of index components between those with different granularity(2 mm and 3-5 mm)and different decocting time(30 min and 60 min),but the extraction rate of index components was relatively high when the granularity of powder was 2 mm and decocting time was 30 min.With the increase of water content,the concentration of index components and the extraction rate were increased in varying degrees.A certain proportion of methanol aqueous solution was used as the resolvent before content determination of the reference sample of Houpu Wenzhong Decoction,which could take into account both the spectral information of water-soluble components and fat-soluble components in the prescription,and help to display the overall information of the prescription’s chemical components more comprehensively.At the same time,the boiling and dispersing classical prescriptions in the Catalogue of Ancient Classical Prescriptions(the first batch)were collected and summarized in this study;the key influencing factors of decocting process were analyzed from different angles,and preliminary research suggestions were put forward,so as to provide a certain direction and reference for the establishment of quality standard of Houpu Wenzhong Decoction,as well as for the development,research and clinical application of boiling and dispersing classical prescriptions.

关 键 词：厚朴温中汤 经典名方 物质基准 煮散 制备工艺 

分 类 号：R28[医药卫生—中药学]