诺丽发酵液的化学成分研究  

Chemical constituents from fermented Noni juice

作　　者：张斌[1,2] 周学明 赵婷[1,2] 王佳莉 李苗苗 吕森 周琴 陈光英 ZHANG Bin;ZHOU Xue-ming;ZHAO Ting;WANG Jia-li;LI Miao-miao;LYU Sen;ZHOU Qin;CHEN Guang-ying(Key Ijnboraiory of Tropical Medicinal Plant Chemistry of Ministry of Education,Hainan Normal University,Haikou 571158,China;Key Laboratory of Tropical Medicinal Plant Chemistry of Hainan Province,College of Chemistry arul Chemical Engineering,Hainan formal Unitrrsily,Haikou 571158,China)

机构地区：[1]海南师范大学热带药用植物化学教育部重点实验室,海南海口571158 [2]海南省热带药用植物化学重点实验室,海南师范大学化学化工学院,海南海口571158

出　　处：《中国中药杂志》2019年第18期4015-4020,共6页China Journal of Chinese Materia Medica

基　　金：海南省重点研发计划项目(ZDYF2019116);海南省自然科学基金项目(217114);教育部创新团队项目(IRT-16R19);中国民族医药重点实验室(中央民族大学)教育部开放基础项目(KLEM-KF2018Y04);2018年海南省研究生创新科研课题(Hys2018-210)

摘　　要：研究诺丽发酵液的化学成分。综合运用硅胶柱色谱、Sephadex LH-20、高效液相色谱等技术进行分离,从诺丽发酵液的乙酸乙酯部分分离得到10个化合物,并运用NMR技术对分离得到的化合物进行结构鉴定。分别鉴定为:norimester(1),cyclo(R-Pro-S-Phe)(2),cyclo-(L-Pro-L-Leu)(3),trans-cyclo-(D-tryptophanyl-L-tyrosyl)(4),cyclo(L-Trp-N-methyl-L-Ala-)(5),4-allyl-2-hydroxyphenyl 1-O-β-D-apiosyl-(1→6)-β-D-glucopyranoside(6),2-methoxy-4-vinylphenylβ-D-apiofuranosyl-(1→6)-β-D-glucopyranoside(7),(+)-lirioresinol A(8),(±)-syringaresinol(9)和2-formyl-1H-pyrrole-1-butanoic acid(10)。并对所有化合物进行了抗菌和α-葡萄糖苷酶抑制活性测试。化合物1为新化合物,所有化合物均为首次从诺丽发酵液中分离得到。化合物1具有较好的α-葡萄糖苷酶抑制活性[IC50为(4.22±0.03)μmol·L^-1]。The present work was launched to study the chemical constituents from fermented Noni juice.Ten compounds were isolated by using silicagel,Sephadex LH-20 and pre-HPLC.The structures of these compounds were identified by 1D and 2D NMR.Their structures were established as norimester(1),cyclo(R-Pro-S-Phe)(2),cyclo-(L-Pro-L-Leu)(3),trans-cyclo-(D-tryptophanyl-Ltyrosyl)(4),cyclo(L-Trp-N-methyl-L-Ala-)(5),4-allyl-2-hydroxyphenyl 1-O-β-D-apiosyl-(1→6)-β-D-glucopyranoside(6),2-methoxy-4-vinylphenylβ-D-apiofuranosyl-(1→6)-β-D-glucopyranoside(7),(+)-lirioresinol A(8),(±)-syringaresinol(9),and2-formyl-1 H-pyrrole-1-butanoic acid(10).All compounds were tested for their antibacterial activity andα-glucosidase inhibitory activity.Compound 1 was a new compound.All compounds were isolated from fermented Noni juice for the first time.Compound 1 showed significant inhibitory activity againstα-glucosidase[IC50 value of(4.22±0.03)μmol·L^-1].

关 键 词：诺丽发酵液 化学成分 结构鉴定 α-葡萄糖苷酶抑制活性 

分 类 号：R28[医药卫生—中药学]