锶磷灰石@壳聚糖-聚氧化乙烯@明胶复合支架制备及其生物性能探究  

Preparation and biological properties of Strontium hydroxyapatite @Chitosan-PEO@Gelatin composite scaffold

作　　者：刘乐云 冯诗璇 王耐艳 郑莹莹[1] LIU Leyun;FENG Shixuan;WANG Naiyan;ZHENG Yingying(School of Sciences,Zhejiang Sci-Tech University,Hangzhou 310018,China;College of Materials and Textiles,Zhejiang Sci-Tech University,Hangzhou 310018,China)

机构地区：[1]浙江理工大学理学院,杭州310018 [2]浙江理工大学材料与纺织学院,杭州310018

出　　处：《浙江理工大学学报（自然科学版）》2019年第6期776-785,共10页Journal of Zhejiang Sci-Tech University(Natural Sciences)

基　　金：国家自然科学基金项目(51603187)

摘　　要：为了模拟自然骨的成分以及进一步提高材料的生物活性,制备羟基磷灰石@壳聚糖@明胶和锶磷灰石@壳聚糖@明胶复合支架,并对两种材料的生物性能进行表征。通过同轴电纺制备核壳结构的壳聚糖@明胶纤维;并采用化学沉积方法,在壳聚糖@明胶纤维表面沉积羟基磷灰石或锶磷灰石颗粒。结果显示:沉积锶磷灰石后,支架仍然能保持利于细胞生长的网状结构;细胞毒性实验和增殖实验结果表明,MG-63细胞在支架材料上的存活率均超过70%;与羟基磷灰石@壳聚糖@明胶复合支架相比,锶磷灰石@壳聚糖@明胶复合支架的细胞存活率更高,表明该材料确实促进细胞的生长和活性。因此锶磷灰石@壳聚糖@明胶复合支架有良好的骨组织工程应用前景。In order to simulate the composition of natural bone and further improve the biological activity of the materials,hydroxyapatite@chitosan@gelatin composite scaffolds and strontium apatite@chitosan@gelatin composite scaffolds were prepared and the biological properties of the two materials were then characterized.Chitosan@gelatin fibers with core-shell structure were prepared through coaxial electrospinning.Meanwhile,hydroxyapatite or Strontium apatite particles were deposited on the surface of chitosan@gelatin fibers by chemical deposit method.The results showed that after the deposition of strontium apatite on the surface of chitosan@gelatin fibers,the scaffolds could still maintain the network structure in favor of cell growth.Cytotoxicity test and proliferation test indicated that the survival rate of MG-63cells on the scaffold exceeded 70%.Compared with HAP@CS@GEL composite scaffolds,SrHAP@CS@GEL composite scaffolds had higher cell survival rate,indicating that the materials can promote cell growth and activity.Therefore,the SrHAP@CS@GEL composite scaffolds have good promising application in bone tissue engineering.

关 键 词：壳聚糖 明胶 同轴电纺 锶磷灰石 骨组织工程 细胞培养 

分 类 号：Q28[生物学—细胞生物学]