不同剂量X射线照射对介入放射工作人员离体外周血淋巴细胞凋亡率的影响  

Effects of different doses of X-ray irradiation on the apoptosis rate of peripheral blood lymphocytes in interventional radiology workers

作　　者：张琳[1] 刘东仁 侯殿俊[1] 李洁清[1] 牛菲[1] 刘伟[1] ZHANG Lin;LIU Dongren;HOU Dianjun;LI Jieqing;NIU Fei;LIU Wei(Institute of Radiation Medicine Shandong Academy of Medical Sciences,Shandong First Medical University,Jinan 250062 China;Shandong Province Rehabilitation Research Center)

机构地区：[1]山东省医学科学院放射医学研究所山东第一医科大学,山东济南250062 [2]山东省运动康复研究中心

出　　处：《中国辐射卫生》2019年第4期368-370,共3页Chinese Journal of Radiological Health

基　　金：山东省医药卫生科技发展计划面上项目(2016WS0518);山东省自然科学基金联合专项(ZR2016YL017)

摘　　要：目的比较介入放射工作人员和健康非放射工作人员离体外周血在受到不同剂量X射线照射后,血液中淋巴细胞凋亡率、Fas表达率的差异。方法采集上述两类人员清晨空腹外周血,分为对照组和照射组,对照组不照射X射线,照射组分别给予1、2、4 Gy剂量的X射线照射,剂量率为1.0 Gy/min,照射后分离提取淋巴细胞、染色培养,12 h后采用流式细胞仪检测淋巴细胞凋亡率和Fas表达率,用flowjo和SPSS软件处理分析数据。结果两类人员离体外周血中T淋巴细胞早期、晚期及总凋亡率组间差异无统计学意义(t=4.58,P>0.05),Fas表达率差异有统计学意义(t=6.75,P<0.05)。每一类人员各剂量组间T淋巴细胞早期、晚期、总凋亡率及Fas表达率组间差异无统计学意义(t=3.96,P>0.05)。结论不同剂量X射线照射对介入放射工作人员、健康非放射工作人员离体外周血T淋巴细胞凋亡率及Fas表达率没有显著性影响。Objective To compare the differences of apoptosis rate of lymphocyte and Fas expression rate in the blood of isolated peripheral blood of interventional radiological workers and healthy non-radiological workers after different doses of X-ray irradiation.Methods To collect the above two groups of peripheral blood on an empty stomach in the morning and blood were divided into control group and the irradiation group.Control group don't illuminate X-ray,irradiation group were given 1,2,4 Gy X-ray irradiation dose respectively.The dose rate was 1.0 Gy/min,lymphocytes were isolated and cultured after irradiation.After 12 hours,lymphocyte apoptosis rate and Fas expression rate were detected by flow cytometry.The data were processed by flowjo and SPSS software.Results There was no significant difference in the early,late and total apoptotic rate of T-lymphocytes between the two groups(t=4.58,P>0.05).The difference of Fas expression rate was statistically significant between the two groups(t=6.75,P<0.05).There was no significant difference in the early,late and total apoptotic rate of T-lymphocytes and Fas expression rate in each group(t=3.96,P>0.05).Conclusion Different doses of X-ray irradiation had no significant effect on the apoptosis rate of peripheral blood T lymphocytes and Fas expression rate in interventional radiology workers and healthy non-radiology workers.

关 键 词：低剂量电离辐射 介入放射工作人员 淋巴细胞 凋亡 

分 类 号：X591[环境科学与工程—环境工程]