天麻素穴位注射对戊四氮致痫模型大鼠脑内海马区凋亡调控因子Bcl-2、Caspase-3表达的影响  

Effect of Gastrodin Acupoint Injection on Expressions of Bcl-2 and Caspase-3 in Hippocampus of Rats with Pentaerytetrazine-Induced Epilepsy

作　　者：肖飞 刘鹏 程光宇 毕海洋 于楠楠 XIAO Fei;LIU Peng;CHENG Guangyu;BI Haiyang;YU Nannan(The First Affiliated Hospital of Heilongjiang University of Chinese Medicine,Harbin 150040,China)

机构地区：[1]黑龙江中医药大学附属第一医院

出　　处：《针灸临床杂志》2019年第10期66-70,共5页Journal of Clinical Acupuncture and Moxibustion

基　　金：黑龙江省自然科学基金面上项目，编号:H2016063

摘　　要：目的:探究天麻素穴位注射对戊四氮致痫模型大鼠行为学及脑内海马区凋亡调控因子Bcl-2、Caspase-3表达的影响。方法:通过腹腔注射戊四氮建立癫痫大鼠模型。按随机数字表法分为空白组(N组)、模型组(M组)、穴注组(AI组)及西药组(SV组),每组各30只。观察记录大鼠行为学变化,并于致痫后处死大鼠,取脑组织海马区以SABC法检测Bcl-2、Caspase-3阳性细胞数,并以RT-PCR及Western Blot分别检测Bcl-2、Caspase-3 mRNA及其蛋白的表达。结果:除N组外,其余各组大鼠均有不同程度的痫性发作。与M组比较,AI组及SV组均可提升癫痫大鼠脑组织海马区中Bcl-2阳性细胞数、基因及蛋白的表达,差异显著(P<0.05),而AI与SV组比较无明显差异(P>0.05);同时AI组及SV组可有效降低癫痫大鼠脑组织海马区中Caspase-3阳性细胞数、基因及蛋白的表达,与M组比较差异显著(P<0.05),且AI与SV组比较无明显差异(P>0.05)。结论:天麻素穴位注射可有效抑制癫痫模型大鼠痫性发作,并可显著上调致痫模型大鼠脑组织海马区中抗凋亡因子Bcl-2的表达,下调其凋亡因子Caspase-3的表达,此治法治疗癫痫疗效确切。Objective:To investigate the effect of gastrodin acupoint injection on the behavior and the expressions of Bcl-2 and Caspase-3,the apoptotic regulatory factors,in the hippocampus of rats with pentotetrazine-induced epilepsy.Methods:The rat model of epilepsy was established by intraperitoneal injection of Pentaerythritol.The rats were randomly divided into the normal group,the model group,the acupoint injection group and the western medication group,with 30 rats in each group.The behavioral changes of rats were observed and recorded,and rats were sacrificed after epilepsy.The numbers of positive cells of Bcl-2 and Caspase-3 in the hippocampal area of brain tissue were detected by SABC method,and the mRAN and protein expressions of Bcl-2 and Caspase-3 were detected by RT-PCR and Western Blot method.Results:The rats in the modeling groups had different degrees of seizures.The numbers of Bcl-2 positive cells,gene and protein expressions in hippocampal area of epileptic rats were significantly increased in the acupoint injection group and in the western medication group compared to those in the model group(P<0.05);of which there was no significant difference between the acupoint injection group and the western medication group(P>0.05).At the same time,the number of caspase-3 positive cells,gene and protein expression in the hippocampal area of epileptic rats were significantly decreased in the acupoint injection group and in the western medication group compared to those in the model group(P<0.05);of which there was significant difference between the acupoint injection group and the western medication group(P>0.05).Conclusion:Gastrodin acupoint injection can effectively inhibit epileptic seizures in the rats,and it can significantly up-regulate the expression of anti-apoptotic factor Bcl-2 and down-regulate the expression of the apoptotic factor Caspase-3 in the hippocampus of epileptic model rats.The therapy has a positive therapeutic effect on epilepsy.

关 键 词：天麻素 穴位注射 癫痫 BCL-2 Caspase-3 

分 类 号：R246.6[医药卫生—针灸推拿学]